Cutting Edge: Stimulation of CD28 with B7-2 Promotes Focal Adhesion-Like Cell Contacts Where Rho Family Small G Proteins Accumulate in T Cells

Unless a costimulatory signal is provided, TCR recognition of Ag bound to the MHC is insufficient to induce optimal T cell proliferation or the production of IL-2. Here we show that the stimulation of CD28, a T cell costimulatory receptor, by a specific Ab increases F-actin contents in T cells. The...

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Published in:The Journal of immunology (1950) 1998-01, Vol.160 (1), p.24-27
Main Authors: Kaga, Shuji, Ragg, Scott, Rogers, Kem A, Ochi, Atsuo
Format: Article
Language:English
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Summary:Unless a costimulatory signal is provided, TCR recognition of Ag bound to the MHC is insufficient to induce optimal T cell proliferation or the production of IL-2. Here we show that the stimulation of CD28, a T cell costimulatory receptor, by a specific Ab increases F-actin contents in T cells. The interaction between T cells and B7–2-transfected Chinese hamster ovary cells expressing the CD28 ligand leads to the rearrangement of the actin cytoskelton in the region of cell-cell contact. Within the Rho family of G proteins, Rac1, but not Rho, translocates to the sites of cell-cell contact where Tailin also accumulates. These results indicate that the interaction between B7–2 and CD28 establishes a focal adhesion-like cell contact between T cell and APCs. The results also suggest that CD28 signaling is primarily transduced by a cytoskeletal rearrangment/signaling pathway mediated by the Rho family G proteins.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.160.1.24