Cutting Edge: Absence of Expression of RAG1 in Peritoneal B-1 Cells Detected by Knocking into RAG1 Locus with Green Fluorescent Protein Gene

It has been proposed that Ig gene rearrangement in the peritoneal cavity (Pc) B-1 cells might be involved in autoantibody generation. To study possible secondary B cell maturation, we prepared mice carrying a target integration of gfp gene into a rag1 locus (rag1/gfp mice). The GFP+ cells express ra...

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Bibliographic Details
Published in:The Journal of immunology (1950) 1999-12, Vol.163 (12), p.6355-6359
Main Authors: Kuwata, Naomi, Igarashi, Hideya, Ohmura, Takafumi, Aizawa, Shinichi, Sakaguchi, Nobuo
Format: Article
Language:English
Subjects:
Animals
B-Lymphocytes - metabolism
Crosses, Genetic
DNA Nucleotidyltransferases - biosynthesis
DNA Nucleotidyltransferases - deficiency
DNA Nucleotidyltransferases - genetics
Gene Expression Regulation - immunology
Genes, RAG-1 - immunology
Genetic Vectors - chemical synthesis
Genetic Vectors - metabolism
Green Fluorescent Proteins
Homeodomain Proteins - biosynthesis
Homeodomain Proteins - genetics
Integrases
Luminescent Proteins - genetics
Mice
Mice, Mutant Strains
Mutagenesis, Insertional
Peritoneum - cytology
Recombinases
RNA, Messenger - biosynthesis
Signal Transduction - genetics
Signal Transduction - immunology
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Summary:It has been proposed that Ig gene rearrangement in the peritoneal cavity (Pc) B-1 cells might be involved in autoantibody generation. To study possible secondary B cell maturation, we prepared mice carrying a target integration of gfp gene into a rag1 locus (rag1/gfp mice). The GFP+ cells express rag1 mRNA and are undergoing Ig gene rearrangement. RAG1 expression was studied in Pc B-1 cells to detect cells during the stage of Ig gene rearrangement. In contrast to previous reports, Pc B-1 cells did not show RAG1 expression in adolescent or elderly mice. RAG1 expression was not induced in Pc B-1 cells in vivo after stimulation by oral or i.p. administration of LPS. Our results suggest that RAG1 expression in Pc B-1 cells is inhibited for a long period under normal condition and that this suppression is an essential state which maintains allelic exclusion of Ig genes.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.163.12.6355