Bactericidal Properties of Group IIA and Group V Phospholipases A2

Group V phospholipase A(2) (PLA(2)) is a recently characterized 14-kDa secretory PLA(2) of mammalian heart and macrophage-derived cells. Group IIA PLA(2), which is structurally close to group V PLA(2), has been shown to kill Gram-positive bacteria in vitro and to prevent symptoms of Gram-positive in...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2001-03, Vol.166 (6), p.4029-4034
Main Authors: Gronroos, Juha O, Laine, Veli J. O, Janssen, Marcel J. W, Egmond, Maarten R, Nevalainen, Timo J
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Cell Line
Culture Media
Cytotoxins - pharmacology
Drug Resistance, Microbial
Enterococcus faecium - drug effects
Enterococcus faecium - growth & development
Escherichia coli - drug effects
Escherichia coli - growth & development
Gram-Positive Bacteria - drug effects
Gram-Positive Bacteria - growth & development
Group II Phospholipases A2
Group V Phospholipases A2
Humans
Listeria monocytogenes - drug effects
Listeria monocytogenes - growth & development
Microbial Sensitivity Tests
Phospholipases A - pharmacology
Rats
Recombinant Proteins - pharmacology
Salts - metabolism
Serum Albumin, Bovine - metabolism
Staphylococcus aureus - drug effects
Staphylococcus aureus - growth & development
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Summary:Group V phospholipase A(2) (PLA(2)) is a recently characterized 14-kDa secretory PLA(2) of mammalian heart and macrophage-derived cells. Group IIA PLA(2), which is structurally close to group V PLA(2), has been shown to kill Gram-positive bacteria in vitro and to prevent symptoms of Gram-positive infection in vivo. We studied the antibacterial properties of fully active recombinant rat group IIA and V PLA(2)s. Both group IIA and V PLA(2)s were highly bactericidal against Gram-positive bacteria, including methicillin-resistant staphylococci and vancomycin-resistant enterococci. Only high concentrations of group IIA PLA(2) showed some bactericidal effect against the Gram-negative bacterium Escherichia coli. Our results confirm that group IIA PLA(2) is a potent antibacterial enzyme against Gram-positive bacteria. Moreover, we show here that group V PLA(2) is a novel antibacterial mammalian protein, but is less potent than group IIA PLA(2). Both enzymes may be considered as future therapeutic agents against bacterial infections.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.166.6.4029