Analysis of VpreB Expression During B Lineage Differentiation in λ5-Deficient Mice

The VpreB/λ5 surrogate L chain complex is an essential component of the pre-B cell receptor, the expression of which serves as an important checkpoint in B cell development. Surrogate L chains also may serve as components of murine pro-B cell receptors whose function is unknown. We have produced two...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2001-10, Vol.167 (7), p.3734-3739
Main Authors: Stephan, Robert P., Elgavish, Eynav, Karasuyama, Hajime, Kubagawa, Hiromi, Cooper, Max D.
Format: Article
Language:English
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Summary:The VpreB/λ5 surrogate L chain complex is an essential component of the pre-B cell receptor, the expression of which serves as an important checkpoint in B cell development. Surrogate L chains also may serve as components of murine pro-B cell receptors whose function is unknown. We have produced two new mAbs, R3 and R5, that recognize a different VpreB epitope than the one recognized by the previously described VP245 anti-mouse VpreB Ab. These Abs were used to confirm the expression of surrogate L chains on wild-type pro-B and pre-B cell lines. Although undetectable on the cell surface, VpreB was found to be normally expressed within B lineage cells of λ5-deficient mice. Nevertheless, VpreB expression was extinguished at the B cell stage of differentiation in these mice. The normal pattern of VpreB expression in λ5-deficient mice excludes an essential role for pro-B and pre-B cell receptors in VpreB regulation.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.167.7.3734