FPLC in a pipette tip (P3297)

• Published in: The Journal of immunology (1950) 2013-05, Vol.190 (1_Supplement), p.211-211.6
• Main Authors: Mundodi, Suparna, Rishi, Porecha, Murray, Anderson, Nicole, Rapicavoli
• Format: Article
• Language: English
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.190.Supp.211.6

Molecular characterization of the immune response and how this process is implemented requires the purification of native proteins and antibodies. There are numerous purification methods to isolate proteins of interest, but these methods usually require multiple steps to attain the level of purity required for most studies. The Rainin PureSpeed Protein Purification System utilizes pipette tips containing purification resin at their distal end interfaced with an E4 XLS pipette to simplify enrichment procedures for virtually all proteins. The E4 XLS pipette, when set up within a 96-deepwell plate, is able to carry out semi-automated purification of antibodies and other native or recombinant proteins. The pipette is able to drive up-and-down sample flow over the immobilized resin matrix for as many as 12 samples in parallel. The ability of the pipette to carry out liquid handling steps enhances laboratory productivity by allowing an investigator to step away from the apparatus until the next step in the protocol. This new, innovative technology from Rainin Instrument is available with pipette tips containing protein A, protein G, Ni-IMAC, or one of four different ion exchange resins, which allow for multiple options for protein purification. Finally, the PureSpeed System functions well in advanced applications such as protein and chromatin immunoprecipitation, offering speed and versatility relative to agarose and magnetic bead technologies.