Probiotic preparation modulate immune system response to food proteins (IRC10P.418)

• Published in: The Journal of immunology (1950) 2015-05, Vol.194 (1_Supplement), p.196-196.16
• Main Authors: Zlotkowska, Dagmara, Wasilewska, Ewa
• Format: Article
• Language: English
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.194.Supp.196.16

Probiotics are microorganisms which have been reported to have beneficial effect on human health to the different extent. Presented study uses probiotic preparation (one commercial were used) to modulate mucosal immune system response to food allergens beta-lactoglobulin and ovalbumin. Mice were orally fed with 5x107 CFU DICOFLOR® by 24 days. Then mice were orally immunized with antigen on 10, 17 and 24 day of experiment. At 35 days post-immunization, significant decreases in serum Ag-specific IgG and IgA titers were observed: 2^6 and 2^4 for β-LG immunized mice and 2^7 and 2^2 for OVA-immunized mice, respectively . Specific IgA titer in fecal extracts was slightly higher than that in groups not supplemented with DICOFLOR®. On 48th day of the experiment, DTH test was performed. No response was observed 24 and 48 hours after DTH challenge by groups supplemented with probiotics preparation. Lymphocytes from spleen, head and neck lymph nodes, Peyer’s patches and mesenteric lymph nodes were phenotyped for CD8+, CD4+and CD4+CD8+ T cells. In Peyer’s patches, mice immunized β-LG and supplemented with probiotics showed elevated numbers of CD4+CD8+ and CD8+ T cell subsets compared to β-LG group not supplemented with probiotic. Similar results were found for OVA group. Results show that probiotics has potential to modulate immune response to food allergens. Those potential could be used to prevent symptoms of allergy to food allergens.