Examining the function of chemokine receptor deficient-monocytes during infection with Nippostrongylus brasiliensis

• Published in: The Journal of immunology (1950) 2016-05, Vol.196 (1_Supplement), p.205-205.15
• Main Authors: Barnes, Mark A, Wang, Spencer, Lo, David, Nair, Meera G
• Format: Article
• Language: English
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.196.Supp.205.15

Nippostrongylus brasiliensis (Nb) is a murine helminth that acutely infects the lungs and the small intestine, and induces a strong Th2 immune response, which includes contributions from innate immune cells such as monocytes. CCR2, the receptor for MCP1, which is expressed primarily on monocytes, was predictably important for monocyte recruitment during Nb infection. However, it was also shown to be necessary to control hemorrhaging as CCR2−/− mice had more blood in the bronchoalveolar lavage fluid compared to wild-type (WT) mice. In addition, CCR2−/− mice produced higher egg burden and more viable worms based on parasite ATP assays. Signaling through CX3CR1, another receptor expressed on monocytes provoked the opposite phenotype. CX3CR1 was not crucial for cellular recruitment after infection, but Nb from CX3CR1−/− mice produced less eggs and were less viable than Nb from WT mice. To investigate potential mechanisms by which monocytes influence worm viability, infective stage Nb (L3) were co-cultured with sorted Ly6c+ monocytes from CX3CR1 WT or knockout (ko) mice. After 5 days in culture, Nb incubated with ko Ly6c+ cells were less motile than Nb cultured with WT Ly6c+ cells. Taken together, these data indicate that CCR2-dependent infiltrating monocytes control inflammation and promote worm killing, while CX3CR1 signaling in monocytes promotes worm viability potentially by influencing worm motility.