Effects of cell population rate of rat bone marrow derived mast cell on protein constituents analyzed by 2 dimensional electrophoresis

• Published in: The Journal of immunology (1950) 2017-05, Vol.198 (1_Supplement), p.211-211.8
• Main Authors: Cho, Sung-Weon, Oh, Haejin, Shin, Kyung Sook
• Format: Article
• Language: English
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.198.Supp.211.8

Mast cells are the immune cells for defense from infecting organism including helminthes. Rat bone marrow dervied mast cells (rBMMC) are the proliferating cells that are induced from rat hematopoietic stem cells (rHSC) cultured in the presence of both IL-3 and stem cell factor (SCF). rBMMC are suggested as the useful cells for the treatment of clonorchiaiss with the results of rBMMC effects on rat clonorchiasis in vivo. The effects of cell population rate on rHSC for rBMMC preparation were investigated by analysing protein constituents using two dimensional electrophoresis (2-DE) with silver staining visualization. The cells in 4 cell population rate conditions were analyzed with ordinary cell population rate (1.1 × 105/ml, O-CPR), high cell population rate (2.2 × 105/ml, H-CPR), very high cell population rate (5.5 × 105/ml, VH-CPR), and very high heavy cell population rate (11.1 × 105/ml, VHH-CPR). The spot ditribution were described as the patterns that have two main group regions with spot dozens, high molecular weight and low pH (HL), and low molecular weight and high pH (LH). The patterns were comparable among the four conditions of cell population rate. However, in comparison between individual spot, the density of each spot was observed in remarkable difference. The results were suggested that the treatment effects of rBMMC on rat clonorchiasis would be dependent on culture condition of cell population rate.