High-dimension flow cytometry reveals comprehensive deviations in immunophenotypes associated with non-response to melanoma immunotherapies

Immunotherapies have revolutionized the treatment of many cancers, but most patients fail to respond. To investigate immune phenotypes associated with patient response, we assessed 68 unique peripheral blood markers using high-dimension flow cytometry. Samples from 41 metastatic melanoma patients tr...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2019-05, Vol.202 (1_Supplement), p.194-194.21
Main Authors: Woods, David M, Laino, Andressa, Winters, Aidan, Alexandre, Jason, Weber, Jeffrey, Chattopadhyay, Pratip
Format: Article
Language:English
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Summary:Immunotherapies have revolutionized the treatment of many cancers, but most patients fail to respond. To investigate immune phenotypes associated with patient response, we assessed 68 unique peripheral blood markers using high-dimension flow cytometry. Samples from 41 metastatic melanoma patients treated with αPD1 or αCTLA4 and 12 healthy donors (HD) were evaluated. To avoid a loss of higher order data, we used a novel computational approach, CytoBrute, to analyze the data through semi-comprehensive Boolean gating. Lineages (e.g. CD3+CD4+) were evaluated for all possible combinations for up to 15 markers. In αPD1 treated patients 1,051 immune signatures differentiated patient response at baseline with a p-value
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.202.Supp.194.21