Oral administration of Sargassum horneri extract containing Mojabanchromanol attenuates PM-exacerbated allergic airway response in murine model of asthma

• Published in: The Journal of immunology (1950) 2020-05, Vol.204 (1_Supplement), p.65-65.29
• Main Authors: Youngheun, Jee, Herath, Kalahe Hewage Iresha Nadeeka Madushani, Kim, Hyo Jin, Jeon, You Jin, Kim, Hyun Jung
• Format: Article
• Language: English
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.204.Supp.65.29

Epidemiological studies indicate correlation with particulate matter (PM) and the pathogenesis of airway inflammatory lung diseases. We sought to identify whether Sargassum horneri ethanol extract containing mojabanchromanol (SHE) confers maximal health benefit against PM exacerbated allergic asthma. Using BALB/c mice undergoing sensitization and challenge with ovalbumin (OVA, 10 μg) we developed a PM exacerbated mouse model of asthma. After 2 weeks of sensitization, mice were challenged with PM (5 mg/m3) for 7 days consecutively together with oral administration of SHE (200, 400 mg/Kg), Prednisone (5mg/Kg) or PBS. SHE restrained Th2 polarization by attenuating transcription factors GATA3 and Signal transducer and activator of transcription (STAT) 5. This resulted in mitigated eosinophil infiltration in airway and balf and secretion of Th2 cytokines interleukin (IL)-4, IL-5, IL-13 in lung homogenates of PM exacerbated asthmatic mice. Immunoglobulin (Ig) E secretion in serum and activation of mast cells in trachea were markedly attenuated. Concomitantly, SHE further restrained the Th17 cell response in PM exposed allergic mice through attenuating expression of transcription factors RORγT, STAT3 and expression of relevant effector cytokines IL-17a. This further mitigated CD11b+ Ly6G+ neutrophil population in lung. Taken together, SHE significantly suppressed PM exacerbated histopathological changes, hyper secretion of mucus in asthmatic mice. These results suggest that SHE might portend a natural candidate against against PM exacerbated allergic asthma via concomitant inhibiting of Th2/Th17 responses. This research was supported by the Ministry of Oceans and Fisheries, Korea (No. 20170285).