Comparative evaluation of antibacterial efficacy of N-acetylcysteine, Aegle marmelos, and chitosan as intracanal medicaments against Enterococcus faecalis biofilm – An in vitro study

Context: The main objective of root canal treatment is the removal of bacteria. Established medicaments and their combinations have been compromised in efficacy against Enterococcus faecalis, causing the need to explore novel intracanal medicaments. Aim: The aim of the study was to evaluate the anti...

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Bibliographic Details
Published in:Journal of conservative dentistry 2024-12, Vol.27 (12), p.1246-1250
Main Authors: Subiksha, Kannan, Jena, Amit, Sarangi, Priyanka, Mohanty, Saumyakanta, Sahoo, Sajan, Mallick, Rashmi Rekha
Format: Article
Language:English
Subjects:
Acetylcysteine
Antibacterial agents
Comparative analysis
Ethylenediaminetetraacetic acid
Root canal therapy
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Summary:Context: The main objective of root canal treatment is the removal of bacteria. Established medicaments and their combinations have been compromised in efficacy against Enterococcus faecalis, causing the need to explore novel intracanal medicaments. Aim: The aim of the study was to evaluate the antibacterial efficacy of chitosan, N-acetylcysteine (NAC), and Aegle marmelos as intracanal medicaments against E. faecalis biofilm. Materials and Methodology: Minimum inhibitory concentration and susceptibility of medicaments determined. Two hundred and forty dentin disc specimens were prepared and inoculated with E. faecalis for 21 days. Samples were divided into four groups (A - N-acetylcysteine; B - Aegle marmelos; C - Chitosan; D -Control) (n = 60), and two subgroups (n = 30) based on the duration of medicament placed (subgroup 1: 24 h, subgroup 2: 7 days). Thereafter, dentinal shavings were retrieved, incubated in agar plate, visible colonies counted, and statistically analyzed. Results: At 24 h Group C1 exhibited the lowest CFUs, followed by Group A1, Group B, and Group D1. On the 7[sup.th] day, Group B2 showed the lowest CFUs, followed by Group A2, Group C2, and Group D2. Conclusions: Against E. faecalis, NAC has the highest antimicrobial properties closely followed by Aegle marmelos and both provide promising novel possibilities for use as intracanal medicaments. Keywords: Aegle marmelos, antimicrobial, chitosan, Enterococcus faecalis, intracanal medication, N-acetylcysteine
ISSN:2950-4716
0972-0707
2950-4708
DOI:10.4103/JCDE.JCDE_588_24