Comparison and frequency of cell cultured in 48-h and 72-h mitotically treated with phytohemagglutinin-M: Both promise for normal and cancer cells

• Published in: Clinical cancer investigation journal 2020-07, Vol.9 (4), p.110-113
• Main Authors: Abadi, Alireza, Mazloumi, Zeinab, Kolahi, Ali, Sheikhpour, Mojgan, Rezaei-Tavirani, Mostafa, Moradi, Afshin, Ahadi, Mahsa, Aghamaleki, Fatemeh, Safavi, Niloufar, Heidary, Mohammad, Zamani, Mahdi, Rad, Sima, Entezari, Maliheh, Hashemi, Mehrdad, Movafagh, Abolfazl
• Format: Article
• Language: English
• ISSN: 2278-0513; 2278-0513
• DOI: 10.4103/ccij.ccij_6_20

The aim of the present study is hence to comprise of mitotic index outcome in the individuals of normal volunteers involved in two different period of culture with effect of Phytohemagglutinin-M. Comparison of healthy human donors lymphocytes were cultured for 48-h and 72-h and using conventional karyotype technique with minor modification. Mitotic index and cell proliferation frequency were analyzed in the individuals of normal blood donors after preparing of colchicine to cultured metaphases 2 h before cell fixation. The metaphase indices at 48-h and 72-h culture period resulted a perfect yield product. Lymphocytes populations in 72-h laboratory work were more than 48-h cultures (2.73% at 72 h vs. 1.48% at 48 h, P ≤ 0.05). The present research indicates significant comparisons for laboratories and individuals to be set up. With effect of these results, it may help in time-consuming and short time period for patient, as well as fresh suggestion for health-care management decision. Reagent, manual, budget, time for diagnosis, and health-care fulfillment may resolve of this proposal.