Development and evaluation of Diplotaxis muralis (mur) cytoplasmic male sterility system in summer rape

• Published in: Canadian journal of plant science 2003-04, Vol.83 (2), p.261-269
• Main Authors: Riungu, T.C, McVetty, P.B.E
• Format: Article
• Language: English
• Subjects: anthers; backcrossing; cultivars; cytoplasm; cytoplasmic male sterility; flowers; genes; greenhouses; open pollination; pollen; rapeseed; seedling emergence; summer; temperature; testcrosses; vernalization
• ISSN: 0008-4220; 1918-1833
• DOI: 10.4141/P02-093

The Diplotaxis muralis male sterility inducing cytoplasm (mur cytoplasm) and its maintainer genes were transferred from the winter rapeseed male sterile line Mangun mur CMS A-line and the mur CMS maintainer line Mangun mur CMS B-line, respectively, into summer rape lines genetically related to the open-pollinated population cultivars Regent and Karat using standard cross, backcross and test cross breeding procedures. Three genetically identical summer rape mur CMS A-line and B-line pairs, Karat I A-line and B-line, Karat II A-line and B-line and Regent A-line and B line were developed. Flower morphology studies revealed that the mur CMS A-lines had anthers displaying a complete absence of pollen and floral morphology modifications similar to that seen for other CMS systems in summer rape. Seedling emergence, days to first flower, days to maturity, and height of the vernalized mur CMS Mangun A-line and B-line pair and the summer rape mur CMS A-line and B-line pairs were similar among treatments and equal for A-line and B-line pair comparisons within cultivars for plants grown in the greenhouse. There was no evidence of biological benefits or costs associated with the mur cytoplasm. The male sterility for the vernalized Mangun mur CMS A-line and the summer rape mur CMS A-lines was stable at temperatures up to 30/24EC.