CBM1, a Neurospora crassa genomic cosmid library in pAC3 and its use for walking on the right arm of linkage group VII

Gene cloning in Neurospora crassa is often achieved by mutant complementation. However, the cloning strategy sometimes requires the isolation of a specific genomic region (by chromosome walking) before transformation of N. crassa. This is the case, for example, if the gene to be isolated has a non-s...

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Bibliographic Details
Published in:Fungal Genetics Reports 1991-01, Vol.38 (1), p.68-70
Main Authors: SPORENO, E, MACINO, G, CABIBBO, A, BALLARIO, P
Format: Article
Language:English
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Summary:Gene cloning in Neurospora crassa is often achieved by mutant complementation. However, the cloning strategy sometimes requires the isolation of a specific genomic region (by chromosome walking) before transformation of N. crassa. This is the case, for example, if the gene to be isolated has a non-selectable phenotype. Here we specifically describe the construction of the cosmid vector, pAC3, which is designed for direct transformation of N. crassa, its utilization for the construction of a genomic library, and chromosome walking in the region of un-10 on linkage group VII.
ISSN:1941-4765
1941-4765
DOI:10.4148/1941-4765.1449