Implementation of a novel ultra fast metabolic stability analysis method using exact mass TOF-MS

Increasing numbers of compounds requiring stability data means highly optimized methods capable of rapid turnaround are desirable during early discovery. An advanced, generic analytical workflow for metabolic stability has been developed that utilizing ballistic gradient LC (sub 1 min run times), ex...

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Bibliographic Details
Published in:Bioanalysis 2017-02, Vol.9 (4), p.359-368
Main Authors: Manna, Joseph D, Richardson, Samantha J, Moghaddam, Mehran F
Format: Article
Language:English
Subjects:
automated data
automation
ballistic gradient
discovery processing
efficiency
high resolution
mass spectrometry
metabolic stability
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Summary:Increasing numbers of compounds requiring stability data means highly optimized methods capable of rapid turnaround are desirable during early discovery. An advanced, generic analytical workflow for metabolic stability has been developed that utilizing ballistic gradient LC (sub 1 min run times), exact mass TOF-MS (Waters Xevo-G2-XS Q-TOF) and automated data processing (Waters UNIFI software) allowed for rapid integration and interpretation of all data produced, eliminating the need for method development and manual processing. We can analyze and process 96 compounds across two species in quadruplicate in a 24-h period with no method development. An advanced bioanalytical workflow has increased our capacity threefold and reduced our instrument/processing needs threefold.
ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2016-0187