A sensitive assay for ZYAN1 in human whole blood and urine utilizing positive LC-MS/MS electrospray ionization

: A sensitive LC-MS/MS method was developed and validated for estimation of ZYAN1 in human blood/urine. : An analog internal standard IOX2 along with ZYAN1 was quantified using selective reaction monitoring in positive mode. The chromatographic separation was performed by gradient elution with C ana...

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Bibliographic Details
Published in:Bioanalysis 2017-05, Vol.9 (9), p.719-732
Main Authors: Patel, Harilal, Soni, Krunal, Trivedi, Rucha, Heading, Heather, Geue, Jason, Kansagra, Kevinkumar, Gupta, Rahul J, Pandya, Vrajesh B, Srinivas, Nuggehally R, Patel, Pankaj R, Desai, Ranjit C
Format: Article
Language:English
Subjects:
anemia
LC-MS/MS
method validation
pharmacokinetics
prolyl hydroxylase inhibitors
whole blood
ZYAN1
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Summary:: A sensitive LC-MS/MS method was developed and validated for estimation of ZYAN1 in human blood/urine. : An analog internal standard IOX2 along with ZYAN1 was quantified using selective reaction monitoring in positive mode. The chromatographic separation was performed by gradient elution with C analytical column (3 µm, 50 mm × 2.0 mm) with 4-min run time using an acidified mobile phase consisting of ammonium formate and acetonitrile. Protein precipitation enabled extraction of analytes from diluted blood/urine. : Calibration curve of ZYAN1 was linear (2-5000 ng/ml). The recovery of ZYAN1 and IOX2 was between 87 and 104%. Interday and intraday accuracy and precision was found well within the acceptance criteria. : The validated assay was applied for clinical pharmacokinetics of ZYAN1 in healthy volunteers.
ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2017-0014