Cleavage of BLM and Sensitivity of Bloom's Syndrome Cells to Hydroxurea

Patients with Bloom's syndrome (BS) show a strong genetic instability and a predisposition to all types of cancer. Here, we report that the Bloom's syndrome protein (BLM) is cleaved in response to hydroxyurea (HU)- or UVC-induced apoptosis. The appearance and solubility of BLM proteolytic...

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Bibliographic Details
Published in:Cell cycle (Georgetown, Tex.) Tex.), 2002-07, Vol.1 (4), p.268-272
Main Authors: Ababou, Mouna, Dumaire, Virginie, Lécluse, Yann, Amor-Guéret, Mounira
Format: Article
Language:English
Subjects:
Binding
Biology
Bioscience
Calcium
Cancer
Cell
Cycle
Landes
Organogenesis
Proteins
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Summary:Patients with Bloom's syndrome (BS) show a strong genetic instability and a predisposition to all types of cancer. Here, we report that the Bloom's syndrome protein (BLM) is cleaved in response to hydroxyurea (HU)- or UVC-induced apoptosis. The appearance and solubility of BLM proteolytic products differed according to whether proteolysis occurred in response to HU or UVC. One BS cell line homozygous for a null mutation in BLM was resistant to both UVC- and HU-induced apoptosis, while another one expressing a mutated BLM protein was resistant to HU-induced apoptosis but displayed normal sensitivity to UVC. Thus, UVC and HU appear to induce apoptosis through distinct pathways. Key Words: Bloom's syndrome, Apoptosis, Hydroxyurea, Ultraviolet C radiation, RecQ helicase
ISSN:1538-4101
1551-4005
DOI:10.4161/cc.1.4.136