A Novel Malaria Lateral Flow Assay for Detecting Plasmodium falciparum Lactose Dehydrogenase in Busia, Uganda

Rapid diagnostic tests (RDTs) for Plasmodium falciparum commonly detect histidine-rich protein 2 (HRP-2), but HRP-2 deletions are increasingly recognized. We evaluated a prototype test detecting parasite lactate dehydrogenase (pLDH) and compared it to commercially available RDTs at a health facility...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2022-01
Main Authors: Bachman, Christine M., Cate, David M., Grant, Ben, Burkot, Stephen, Mulondo, Jerry, Hsieh, Helen V., Chamai, Martin, Odongo, Bakar, Olwoch, Peter, Nalubega, Mayimuna, Ochokoru, Harriet, Kasozi, Joseph, Ategeka, John, Nichols, Kevin P., Weigl, Bernhard H., Greenhouse, Bryan
Format: Article
Language:English
Online Access:Get full text
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Summary:Rapid diagnostic tests (RDTs) for Plasmodium falciparum commonly detect histidine-rich protein 2 (HRP-2), but HRP-2 deletions are increasingly recognized. We evaluated a prototype test detecting parasite lactate dehydrogenase (pLDH) and compared it to commercially available RDTs at a health facility in Uganda, using quantitative polymerase chain reaction as a gold standard. The prototype pLDH test had a high sensitivity for infections with at least 100 parasites/µL (98%), comparable to HRP-2, and greater than an existing pLDH RDT (89%). Specificity for the prototype test was 99.5%, which is greater than the HRP-2 tests (93–95%). Therefore, the prototype pLDH test may be an attractive alternative malaria diagnostic.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.21-0956