A comparative assessment of the purification techniques for etiolated seedling protoplast of Sorghum bicolor

• Published in: African crop science journal 1997-12, Vol.5 (2)
• Main Author: Umoh, E O
• Format: Article
• Language: English
• Subjects: Etiolated shoots, protoplast purification, sorghum; Tiges etiolees, purification des protoplastes, sorgho
• ISSN: 1021-9730; 2072-6589; 2072-6589
• DOI: 10.4314/acsj.v5i2.27861

Protoplasts of Sorghum bicolor Moench are usually obtained from suspension cells and purified using either the sedimentation or the sucrose floatation methods. Effects of these two methods were assessed using etiolated shoot protoplats of two cultivars of sorghum. Recovery rates were higher in the sedimentation method than in the sucrose floatation method. The ratios of vacuolated to etioplastic in the initial and purified samples of protoplasts were not markedly changed with the sedimentation method. On the other hand, these ratios were largely titled in favour of vacuolated protoplast when the sucrose floatation method was used. This finding could be exploited and an appriopriate purification method selected based on the specific experimental requirements. D'une facon generale, on obtient les protoplastes de Sorghum bicolor Moench a partir des cellules en suspension et purifiees a l'aide des methodes de sedimentation ou de flottaison de sucrose. Les effets de ces deux methodes ont ete evalues en utilisant les protoplastes de la tige etiolee de deux cultivars de sorgho. Les taux de recuperation se sont averes plus eleves dans la methode de sedimentation que dans celle de flottaison du sucrose. La methode de sedimentation n'a pas montre un changement significatif des rapports protoplastes vacuoles sur protoplastes etioles dans les echantillons initiaux et purifies. Par ailleurs, ces proportions ont ete en grande partie titrees en faveur du protoplaste vacuole par la methode de sedimentation du sucrose. On pourrait ainsi exploiter a resultat et selectionner la methode de purification appropriee en se basant sur les besoins experimentaux specifiques.