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Cisplatin Induces Up-Regulation of KAI1, a Metastasis Suppressor Gene, in MCF-7 Breast Cancer Cell Line
Purpose: To investigate the effect of cisplatin on cell toxicity and metastasis through modulation of KAI1 gene expression. Methods: MCF-7cells were incubated with different concentrations of cisplatin for 24 h. RNA was extracted by trizol and cDNA synthesized. KAI1 and TBP were chosen as target and...
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Published in: | Tropical journal of pharmaceutical research 2012-10, Vol.11 (4) |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Purpose: To investigate the effect of cisplatin on cell toxicity and
metastasis through modulation of KAI1 gene expression. Methods:
MCF-7cells were incubated with different concentrations of cisplatin
for 24 h. RNA was extracted by trizol and cDNA synthesized. KAI1 and
TBP were chosen as target and internal control genes, respectively.
Specific primers were designed by primer express software, v.3.0.
KAI1/TBP and gene expression ratio was calculated using the formula,
2-ΔΔCt. Results: Cisplatin exerted a dose-dependent
inhibitory effect on the viability of highly metastatic MCF-7 cells.
KAI1/TBP gene expression ratios were 1.97 ± 0.19 (p < 0.05),
2.96 ± 0.55 (p < 0.05), 9.06 ± 0.27 (p < 0.001) and
12.38 ± 0.88 (p < 0.01) in 10, 20, 50 and 100 μM
concentrations of cisplatin. Conclusion: These findings indicate that
cisplatin can inhibit metastasis by up-regulating KAI1 gene in
MCF-7cells. |
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ISSN: | 1596-5996 1596-9827 |
DOI: | 10.4314/tjpr.v11i4.1 |