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Development and Application of Kinetic Spectrophotometric Method for the Determination of Metronidazole
Purpose: To develop an improved kinetic-spectrophotometric procedure for the determination of metronidazole (MNZ) in pharmaceutical formulations. Methods: The method is based on oxidation reaction of MNZ by hydrogen peroxide in the presence of Fe(II) ions at pH 4.5 (acetate buffer). The reaction was...
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Published in: | Tropical journal of pharmaceutical research 2014-08, Vol.13 (3), p.417 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Purpose: To develop an improved kinetic-spectrophotometric procedure
for the determination of metronidazole (MNZ) in pharmaceutical
formulations. Methods: The method is based on oxidation reaction of MNZ
by hydrogen peroxide in the presence of Fe(II) ions at pH 4.5 (acetate
buffer). The reaction was monitored spectrophotometrically by measuring
the rate of change of absorbance at 317nm. Results: The optimum
operating conditions for reagent concentrations and temperature were
established. Linear calibration curve was obtained in the range of
85.77 - 513.45ng mL-1 with standard deviation from 1.77 to 4.55
%-. The optimized conditions yielded a theoretical detection limit of
15.20 ng mL-1 based on 3.3So criterion. Commonly used excipients and
other additives such as talc, glucose, fructose, lactose, starch,
magnesium stearate, microcrystalline cellulose and several ions were
found not to have interference. Conclusion: The developed method is
sensitive, accurate and reproducible and could be used for routine
analysis of metronidazole in pharmaceutical preparations. |
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ISSN: | 1596-5996 1596-9827 |
DOI: | 10.4314/tjpr.v13i3.16 |