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In vitro Antimicrobial and Antibiofilm Activity of Artocarpus Lakoocha (Moraceae) Extract against Some Oral Pathogens
Purpose: To evaluate the antimicrobial and antibiofilm activity of A. lakoocha extract against oral pathogens by an in vitro method. Methods: The dried powder of the aqueous extract of A. lakoocha was purchased from a Thai traditional drug store. Representative strains of oral pathogens ( Streptococ...
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Published in: | Tropical journal of pharmaceutical research 2014-09, Vol.13 (7), p.1149 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Purpose: To evaluate the antimicrobial and antibiofilm activity of A.
lakoocha extract against oral pathogens by an in vitro method. Methods:
The dried powder of the aqueous extract of A. lakoocha was purchased
from a Thai traditional drug store. Representative strains of oral
pathogens ( Streptococcus mutans ATCC 25175, Streptococcus sobrinus
ATCC 33478, Enterococcus faecalis ATCC 19433, Lactobacillus
fermentum ATCC 14931, Lactobacillus salivarius ATCC 11741,
Aggregatibacter actinomycetemcomitans ATCC 33384, Porphyromonas
gingivalis ATCC 33277, Prevotella intermedia ATCC 25611,
Prevotella nigrescens ATCC 25261, Fusobacterium nucleatum ATCC
25586 and Tanerella forsythia ATCC 43037) were tested for minimal
inhibitory concentration (MIC) and minimal bactericidal concentration
(MBC) using a microdilution technique, as well as by a time kill assay.
Antibiofilm activity was investigated by a 3-[4,
5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide (MTT)
assay. Results: All tested strains were susceptible to A. lakoocha
extract with variable degrees of antimicrobial inhibition. The extract
was effective against both Gram-negative bacteria (Aggregatibacter
actinomycetemcomitans, Porphyromonas gingivalis) and Gram-positive
bacteria (Streptococcus mutans, Streptococcus sobrinus), with MIC
ranging from 0.10 - 0.39 mg/ml and MBC from 0.10 - 3.12
mg/ml. Killing activity depended on time and concentrations of the
extract. The extract acted as a potent antibiofilm agent with dual
actions, preventing biofilm formation and also eradicating the existing
biofilm. Conclusion: A. lakoocha extract possesses compounds with good
antimicrobial properties that may be used for oral infectious diseases
caused by certain oral pathogens associated with dental caries and/or
periodontal diseases. For the application, A. lakoocha extract may be
incorporated in mouthwash or toothpaste. |
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ISSN: | 1596-5996 1596-9827 |
DOI: | 10.4314/tjpr.v13i7.20 |