Evaluation of Gentamicin-Entrapped Solid Lipid Microparticles Formulated with a Biodegradable Homolipid from Capra hircus

• Published in: Tropical journal of pharmaceutical research 2014-09, Vol.13 (8), p.1999
• Main Authors: Kenechukwu, F.C, Umeyor, C.E, Momoh, M.A, Ogbonna, J.D.N, Chime, S.A, Nnamani, P.O, Attama, A.A
• Format: Article
• Language: English
• Subjects: Capra hircus; Gentamicin; Phospholipon® 90 G; Solid lipid microparticles; Solidified reverse micellar solution
• ISSN: 1596-5996; 1596-9827
• DOI: 10.4314/tjpr.v13i8.2

Purpose: To formulate solidified reverse micellar solutions (SRMS)-based solid lipid microparticles (SLMs) using homolipid from Capra hircus , and evaluate its suitability for the delivery of gentamicin. Methods: SLMs were formulated by melt-emulsification using SRMS (15 % w/w Phospholipon® 90G in 35 % w/w Capra hircus), PEG 4000 and gentamicin (1.0, 2.0 and 3.0 % w/w), and characterized with respect to size, morphology, encapsulation efficiency (EE) and pH-dependent stability. In vitro release of gentamicin from the SLMs was performed in phosphate buffer (pH 7.4) while bioevaluation was carried out using clinical isolates of Pseudomonas aeruginosa and Staphylococcus aureus . Results: Stable and discrete SLMs of size range 1.47 ± 0.02 to 3.55 ± 0.09 ìm were obtained. The SLMs showed a biphasic pattern of drug release and exhibited time-dependent and capacity-limited bioactivity. Overall, SLMs containing 2 % w/w SRMS, 3 % w/w gentamicin and PEG 4000 entrapped the highest amount of drug, released 99 % of drug and gave the highest inhibitory zone diameter (IZD) against the organisms within 420 min, while plain gentamicin gave the least. Conclusion: SRMS-based SLMs prepared with homolipid from Capra hircus offers a suitable delivery system for gentamicin.