Mechanism of Klebsiella pneumoniae resistance to carbapenem antibiotics

Purpose: To investigate the mechanism of action of Klebsiella pneumoniae (Kpn) resistance to carbapenem. Methods: The susceptibility of six Klebsiella pneumoniae strains to antibiotics was determined using KB assay. Six isolated strains which were resistant to carbapenem were identified and collecte...

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Bibliographic Details
Published in:Tropical journal of pharmaceutical research 2019-07, Vol.18 (7), p.1519-1526
Main Authors: Chen, Danxia, Zhu, Yan, Hu, Liangshan, Wu, Xiaoyun, Li, Mingyou, Lin, HanHui
Format: Article
Language:English
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Summary:Purpose: To investigate the mechanism of action of Klebsiella pneumoniae (Kpn) resistance to carbapenem. Methods: The susceptibility of six Klebsiella pneumoniae strains to antibiotics was determined using KB assay. Six isolated strains which were resistant to carbapenem were identified and collected using modified Hodge test. The phenotypes of metal enzyme were evaluated by ethylene diamine tetraacetic acid (EDTA) disk diffusion method. The genes for beta-lactamases, including KPC gene, were examined. Results: The six carbapenem-resistant strains of Klebsiella pneumoniae were resistant to imipenem, meropenem and aztreonam, but were sensitive to amikacin, fosfomycin, minocycline, and polymyxin. The six pathogens did not produce metal enzyme, but they produced carbapenemases. Moreover, the six strains partially carried blaTEM or blaSHV gene, but all had blaKPC-2 gene. Conclusion: These results suggest that the pathogens that contain blaKPC-2 gene may be involved in the mechanism of Klebsiella pneumoniae (Kpn) resistance to carbapenem.
ISSN:1596-5996
1596-9827
DOI:10.4314/tjpr.v18i7.23