Vitis vinifera seed extract ameliorates streptozotocin ind uced pancreatic dysfunction by reducing interleukin 1β and up regulation of omentin 1 mRNA

• Published in: Tropical journal of pharmaceutical research 2023-06, Vol.22 (2), p.289-295
• Main Authors: Salah, Karima Bel Hadj, Al-Quwaie, Diana AH, Binothma, Najat, Aljadani, Majidah, Mansoury, Manal MS, Alsubhi, Nouf H, Alrefaei, Ghadeer I, Bayoumi, Ammar A, Ibrahim, Maha, Khattab, Hala AH
• Format: Article
• Language: English
• ISSN: 1596-5996; 1596-9827
• DOI: 10.4314/tjpr.v22i2.10

Purpose: To investigate the effect of   ethanol seed extract (SE) on pancreatic function markers associated with streptozotocin-induced diabetes in rats. Methods: Non-diabetic rats (n = 12) were divided into 2 groups (GRPs); GRP1 served as the control, and GRP2 was administered orally with V. vinifera SE (500 mg/kg). Diabetic rats were divided into 2 GRPs; GRP3 served as the streptozotocin-induced diabetic rats, and GRP4 diabetic rats received V. vinifera SE (500 mg/kg). Blood was collected at the end of 4 weeks, and serum glucose and lipid profile were measured using colorimetric enzymatic kits. Serum insulin, and interleukin-1 beta (IL-1β) levels were quantified using enzyme-linked immunosorbent assay (ELISA) kits. Histopathological alterations in pancreas were determined, while omentin-1 mRNA expression was investigated using quantitative real time-polymerase chain reaction (qRT-PCR). Results: V. vinifera SE protected pancreatic function in diabetic rats. V. vinifera SE significantly increased serum insulin and HDL (p < 0.001), besides significantly decreasing serum glucose, cholesterol, triglyceride, and LDL (p < 0.001). Pathological studies also showed that V. vinifera SE preserved the pancreatic β cells’ shape, size, and cell population in the diabetic GRP. The extract significantly decreased serum IL-1β levels in diabetic rats (p < 0.001). Besides, the data demonstrated a significant up-regulation in omentin-1 mRNA expression in diabetic rats treated with V. vinifera SE. Conclusion: V. vinifera SE has anti-diabetic and anti-hyperlipidemic properties in streptozotocin-induced rat model. The underlying mechanism of action might be a decrease in the pro-inflammatory cytokine (IL-1β) and increase in omentin mRNA. Clinical trials are required to validate these findings