Immunoassays for analysis of mycotoxins [in foods and feeds]

During the past few years, several laboratories have prepared specific antibodies against aflatoxins B , M , B and Q , ochratoxin A, T-2 toxin, and zearalenone. These antibodies were obtained from rabbits after immunizing with various mycotoxin-protein conjugates. With the availability of these anti...

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Bibliographic Details
Published in:Journal of food protection 1984-07, Vol.47 (7), p.562-569
Main Author: Chu, F.S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
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Summary:During the past few years, several laboratories have prepared specific antibodies against aflatoxins B , M , B and Q , ochratoxin A, T-2 toxin, and zearalenone. These antibodies were obtained from rabbits after immunizing with various mycotoxin-protein conjugates. With the availability of these antibodies, specific, simple and sensitive radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) procedures for monitoring mycotoxins and their metabolites in foods, feeds and body fluids have been developed. In this review, details are presented for the preparation of antibodies and the application of RIA and ELISA to determine aflatoxins B and M , ochratoxin A and T-2 toxin in corn, peanuts, milk and other biological fluids. The sensitivity of ELISA for analysis of these mycotoxins in foods varied from 0.1 μg/L for aflatoxin M in milk to 5 μg/kg of aflatoxin B in peanuts. The advantages and disadvantages of ELISA for monitoring mycotoxins in foods and feeds are discussed. In addition, a description of recent progress on simplified clean-up procedures which may increase the sensitivity of immunoassays is presented.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-47.7.562