Identification and toxigenic potential of the industrially important fungi, Aspergillus oryzae and Aspergillus sojae

• Published in: Journal of food protection 2007-12, Vol.70 (12), p.2916-2934
• Main Author: Jorgensen, T.R
• Format: Article
• Language: English
• Subjects: aflatoxins; Aflatoxins - genetics; Aflatoxins - metabolism; Aspergillus; Aspergillus flavus; Aspergillus flavus - classification; Aspergillus flavus - genetics; Aspergillus oryzae; Aspergillus oryzae - classification; Aspergillus oryzae - genetics; Aspergillus parasiticus; Aspergillus sojae; Biological and medical sciences; DNA, Fungal - chemistry; DNA, Fungal - genetics; domestication; Fermentation; fermented foods; Food industries; Food Microbiology; food pathogens; Fundamental and applied biological sciences. Psychology; fungal anatomy; Fungal Proteins - genetics; Genotype; Industrial Microbiology; koji molds; molds (fungi); Phenotype; Phylogeny; Polymorphism, Restriction Fragment Length; Polymorphism, Single-Stranded Conformational; Random Amplified Polymorphic DNA Technique; secondary metabolites; Sequence Analysis, DNA; Sequence Homology; Species Specificity; starter cultures; taxonomic keys; toxigenic strains; wild relatives
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028x-70.12.2916

Mold strains belonging to the species Aspergillus oryzae and Aspergillus sojae are highly valued as koji molds in the traditional preparation of fermented foods, such as miso, sake, and shoyu, and as protein production hosts in modern industrial processes. A. oryzae and A. sojae are relatives of the wild molds Aspergillus flavus and Aspergillus parasiticus. All four species are classified to the A. flavus group. Strains of the A. flavus group are characterized by a high degree of morphological similarity. Koji mold species are generally perceived of as being nontoxigenic, whereas wild molds are associated with the carcinogenic aflatoxins. Thus, reliable identification of individual strains is very important for application purposes. This review considers the pheno- and genotypic markers used in the classification of A. flavus group strains and specifically in the identification of A. oryzae and A. sojae strains. Separation of A. oryzae and A. sojae from A. flavus and A. parasiticus, respectively, is inconsistent, and both morphologic and molecular evidence support conspecificity. The high degree of identity is reflected by the divergent identification of reference cultures maintained in culture collections. As close relatives of aflatoxin-producing wild molds, koji molds possess an aflatoxin gene homolog cluster. Some strains identified as A. oryzae and A. sojae have been implicated in aflatoxin production. Identification of a strain as A. oryzae or A. sojae is no guarantee of its inability to produce aflatoxins or other toxic metabolites. Toxigenic potential must be determined specifically for individual strains. The species taxa, A. oryzae and A. sojae, are currently conserved by societal issues.