Decreased Hyaluronan Concentration during Primary Rhegmatogenous Retinal Detachment

Purpose To evaluate the concentration and molecular weight of hyaluronan (HA) polysaccharides as well as hyaluronidase activity in patients with rhegmatogenous retinal detachment (RRD). Methods Twenty vitreous samples from 20 patients with RRD and 19 samples from 19 patients with idiopathic epiretin...

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Published in:European journal of ophthalmology 2016-11, Vol.26 (6), p.633-638
Main Authors: Kaprinis, Konstantinos, Symeonidis, Chrysanthos, Papakonstantinou, Eleni, Tsinopoulos, Ioannis, Dimitrakos, Stavros A.
Format: Article
Language:English
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Summary:Purpose To evaluate the concentration and molecular weight of hyaluronan (HA) polysaccharides as well as hyaluronidase activity in patients with rhegmatogenous retinal detachment (RRD). Methods Twenty vitreous samples from 20 patients with RRD and 19 samples from 19 patients with idiopathic epiretinal membrane, macular hole, or vitreomacular traction syndrome were collected during surgical management with pars plana vitrectomy. The molecular weight of various HA fragments was assessed using agarose gel electrophoresis. Enzyme-linked immunosorbent assay was employed for the measurement of HA (in μg/mL). Hyaluronidase activity was evaluated using substrate (HA) sodium dodecyl sulfate polyacrylamide gel electrophoresis. Results Agarose gel electrophoresis showed that the vitreous of the control group contained HA of high molecular mass, in contrast with the patient group. Mean HA concentration in the patient group was 50.96 μg/mL and differed significantly from that of the control group, which was 271.81 μg/mL (p
ISSN:1120-6721
1724-6016
DOI:10.5301/ejo.5000802