Isolation and characterization of alginate from Hizikia fusiformis and preparation of its oligosaccharides

In this study, alginate was isolated from the residues of Hizikia fusiformis, after extraction of the fucoidan. The yield, total carbohydrate, uronic acid, ash and moisture of alginate was 2.6% (based on wet material), 85.2, 80.5, 12.1 and 2.2%, respectively. The molecular mass of alginate was calcu...

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Bibliographic Details
Published in:Journal of Applied Glycoscience 2007, Vol.54(2), pp.85-90
Main Authors: Shiroma, R.(Kagoshima Univ. (Japan)), Uechi, S, Tawata, S, Tako, M
Format: Article
Language:English
Subjects:
AISLAMIENTO
ALGAS MARINAS
ALGINATE
alginate oligosaccharide
ALGINATES
ALGINATOS
ALGUE MARINE
AZUCARES ACIDOS
CHEMICAL COMPOSITION
CHEMICAL STRUCTURE
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
ESTRUCTURA QUIMICA
fucoidan
HIDROLISIS
Hizikia fusiformis
HYDROLYSE
HYDROLYSIS
ISOLATION
ISOLEMENT
LIASAS
LYASE
LYASES
MOLECULAR WEIGHT
OLIGOSACARIDOS
OLIGOSACCHARIDE
OLIGOSACCHARIDES
PESO MOLECULAR
POIDS MOLECULAIRE
SEAWEEDS
STRUCTURE CHIMIQUE
SUCRE ACIDE
SUGAR ACIDS
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Summary:In this study, alginate was isolated from the residues of Hizikia fusiformis, after extraction of the fucoidan. The yield, total carbohydrate, uronic acid, ash and moisture of alginate was 2.6% (based on wet material), 85.2, 80.5, 12.1 and 2.2%, respectively. The molecular mass of alginate was calculated to be about 7.0 x 10E4. The sup(1)H- and sup(13)C-NMR and IR spectra of alginate from H. fusiformis were in agreement with those of standard alginate. From the integral of the anomeric proton signals of D-mannuronic (M) and L-guluronic (G) acid residues in the sup(1)H-NMR spectrum, the molar ratio of both residues was calculated to be M: G = 0.53: 1. Eight alginate oligosaccharides were also isolated by gel permeation chromatography after using commercially available enzymes and were characterized by 2 D NMR techniques and ESI-MS spectrometry as delta(4-deoxy-L-erythro-hex-4-enopyranosyluronate) GGGG, deltaGGG, deltaGG, deltaG, deltaMMMM, deltaMMM, deltaMM and deltaM.
ISSN:1344-7882
1880-7291
DOI:10.5458/jag.54.85