Overexpression of a delayed early gene hlg1 of temperate mycobacteriophage L1 is lethal to both M. smegmatis and E. coli

Two genes of temperate mycobacteriophage L5, namely, gp63 and gp64, were hypothesized to be toxic to M. smegmatis. An identical L5 gp64 ortholog (designated hlg1) was cloned from homoimmune mycobacteriophage L1 and characterized at length here. As expected, hlg1 affected the growth of M. smegmatis w...

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Published in:BMB reports 2008-05, Vol.41 (5), p.363-368
Main Authors: Chattoraj, Partho (Bose Institute, Acaharya J. C. Bose Birth Centenary Building, Calcutta, India), Ganguly, Tridib (Bose Institute, Acaharya J. C. Bose Birth Centenary Building, Calcutta, India), Nandy, Ranjan Kumar (National Institute of Cholera and Enteric Diseases, Kolkata, India), Sau, Subrata (Bose Institute, Acaharya J. C. Bose Birth Centenary Building, Calcutta, India), E-mail: sau@bic.boseinst.ernet.in
Format: Article
Language:English
Subjects:
delayed early gene
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli - metabolism
GENE
Gene Expression
GENES
growth retardation
host lethal gene
Microbial Viability - genetics
mycobacteriophage L1
Mycobacteriophages - genetics
Mycobacterium smegmatis - genetics
Mycobacterium smegmatis - growth & development
Mycobacterium smegmatis - metabolism
Plasmids - genetics
Transcription, Genetic
Viral Proteins - genetics
Viral Proteins - physiology
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Summary:Two genes of temperate mycobacteriophage L5, namely, gp63 and gp64, were hypothesized to be toxic to M. smegmatis. An identical L5 gp64 ortholog (designated hlg1) was cloned from homoimmune mycobacteriophage L1 and characterized at length here. As expected, hlg1 affected the growth of M. smegmatis when overexpressed from a resident plasmid. HLG1 (the protein encoded by hlg1) in fact caused growth retardation of M. smegmatis and the region encompassing its 57-114 C-terminal amino acid residues was found indispensable for its growth-retardation activity. Both nucleic acid and protein biosynthesis were severely impaired in M. smegmatis expressing HLG1. Interestingly, HLG1 also affected E. coli almost similarly. This putative delayed early lipoprotein did not participate in the lytic growth of L1.
ISSN:1976-6696
DOI:10.5483/BMBRep.2008.41.5.363