Montelukast protects against testes ischemia/reperfusion injury in rats

• Published in: Canadian Urological Association journal 2013-04, Vol.4 (3), p.174
• Main Authors: Ozturk, Hulya, Ozturk, Hayrettin, Gideroglu, Kaan, Terzi, Hakan, Bugdayci, Guler
• Format: Article
• Language: English
• ISSN: 1920-1214; 1920-1214
• DOI: 10.5489/cuaj.847

Introduction: In this study, we investigate the effect of montelukaston histologic damage induced by testicular torsion-detorsion in rats.Methods: Twenty-one male Sprague-Dawley rats were separatedinto 3 groups, each containing 7 rats. A sham operation was performedin group 1 (control). In group 2 (ischemia-reperfusion [IR]/untreated), 1-hour detorsion of the testis was performed after6 hours of unilateral testicular torsion. In group 3 (I-R/dextroamphetamine),after performing the same surgical procedures as ingroup 2, montelukast was given intraperitoneally. In all experimentalrats, ipsilateral orchiectomies were performed for histologicalexamination and tissue malondialdehyde (MDA), glutathioneand myeloperoxidase assays.Results: Montelukast treatment significantly decreased the I-Rinducedelevation in testes tissue MDA and glutathione levelswere found to be preserved. The level of myeloperoxidase (MPO)activity was significantly increased in the testes tissue of the IR/untreated group. However, in I-R/montelukast treatment groupsignificantly decreased testes tissue MPO level. Histopathologically,the in the group 2 rats, edema, congestion, hemorrhage betweenseminiferous tubules and necrosis of the germinal cells were predominantfeatures in sections. However, most of the specimensin the montelukast treated group 3 showed grades-I and II injury.Additionally, the testicular injury score was lower in group 3 ratscompared with group 2.Conclusion: The current findings demonstrate that the montelukastdecreased the severity of testicular injury by reversing the oxidativeeffects of testes I-R.Introduction : Dans cette étude, nous examinons l’effet du montélukastsur les lésions tissulaires provoquées par torsion-détorsiontesticulaire chez le rat.Méthodologie : Vingt-et-un rats Sprague-Dawley mâles ont étérépartis en trois groupes de 7 rats. Une opération fictive a étéréalisée dans le groupe 1 (groupe témoin). Dans le groupe 2(ischémie-reperfusion / non traité), une détorsion testiculaire d’uneheure a été réalisée après 6 heures de torsion testiculaire unilatérale.Dans le groupe 3 (ischémie-reperfusion / dextroamphétamine),on a administré du montélukast par voie intrapéritonéaleaprès les mêmes interventions chirurgicales que pour le groupe 2.Chez tous les rats, une orchidectomie homolatérale a été effectuéeen vue d’un examen histologique et de mesures du malonaldéhyde,du glutathion et de la myéloperoxidase dans les tissus.Résultats : Le traitement par montélukast a si