Phenobarbital Analysis in Biological Matrix (Blood) by High Performance Liquid Chromatography (HPLC)

In this work, we have tried to contribute to the analysis of phenobarbital in the blood. To do this, we used different analytical techniques such as liquid chromatography (HPLC). The results obtained in the course of our work, we can conclude that: The HPLC method was separate phenobarbital endogeno...

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Bibliographic Details
Published in:International letters of chemistry, physics and astronomy physics and astronomy, 2013-10, Vol.20, p.31-40
Main Authors: Hamza, Ouakouak, Moktar, Ben Mohamed, Mostafa, Ben Chohra, Zeghdaoui, Abdelhamid
Format: Article
Language:English
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Summary:In this work, we have tried to contribute to the analysis of phenobarbital in the blood. To do this, we used different analytical techniques such as liquid chromatography (HPLC). The results obtained in the course of our work, we can conclude that: The HPLC method was separate phenobarbital endogenous blood components, and optimizing the conditions of chromatographic separation as the composition of the mobile phase consisting of 20% acetonitrile + 20% methanol + 60% ammonium acetate buffer. The extraction with diethyl ether to pH = 4; The chromatographic column, microbondapack ZORBAX C18. A system of diode-array UV detection at 254 nm.
ISSN:2299-3843
2299-3843
DOI:10.56431/p-6t05wn