Surface Deposition Method; A Novel Protein Immobilization Procedure on a Sensor Surface

A novel immobilization method, surface deposition method, for a membrane-binding protein on the solid surfaces of the sensor is described. We focused on the hydrophobicity of a membrane-binding region of a membrane-binding protein for this immobilization method. The surface deposition method consist...

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Bibliographic Details
Published in:Denki kagaku oyobi kōgyō butsuri kagaku 2000/11/05, Vol.68(11), pp.882-885
Main Authors: YAMAZAKI, Tomohiko, YASUTAKE, Nozomu, SODE, Koji
Format: Article
Language:English
Subjects:
Amperometric Biosensor
Colorimetric Biosensor
Glucose Dehydrogenase
Membrane Binding Protein
Protein Immobilization Procedure
Pyrroloquinoline Quinone
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Summary:A novel immobilization method, surface deposition method, for a membrane-binding protein on the solid surfaces of the sensor is described. We focused on the hydrophobicity of a membrane-binding region of a membrane-binding protein for this immobilization method. The surface deposition method consists of two steps; the first step is the protein adsorption on support surfaces by electrostatic interaction, and the second step is the deposition of the hydrophobic region of a membrane-binding protein onto the surfaces. The surface deposition method can immobilize a protein on solid surfaces under milder conditions than typical protein immobilization methods such as cross-linking and covalent binding. We also constructed a novel amperometric glucose sensing system employing membrane-binding PQQ glucose dehydrogenase using the surface deposition method.
ISSN:1344-3542
2186-2451
DOI:10.5796/electrochemistry.68.882