Surface Deposition Method; A Novel Protein Immobilization Procedure on a Sensor Surface

• Published in: Denki kagaku oyobi kōgyō butsuri kagaku 2000/11/05, Vol.68(11), pp.882-885
• Main Authors: YAMAZAKI, Tomohiko, YASUTAKE, Nozomu, SODE, Koji
• Format: Article
• Language: English
• Subjects: Amperometric Biosensor; Colorimetric Biosensor; Glucose Dehydrogenase; Membrane Binding Protein; Protein Immobilization Procedure; Pyrroloquinoline Quinone
• ISSN: 1344-3542; 2186-2451
• DOI: 10.5796/electrochemistry.68.882

A novel immobilization method, surface deposition method, for a membrane-binding protein on the solid surfaces of the sensor is described. We focused on the hydrophobicity of a membrane-binding region of a membrane-binding protein for this immobilization method. The surface deposition method consists of two steps; the first step is the protein adsorption on support surfaces by electrostatic interaction, and the second step is the deposition of the hydrophobic region of a membrane-binding protein onto the surfaces. The surface deposition method can immobilize a protein on solid surfaces under milder conditions than typical protein immobilization methods such as cross-linking and covalent binding. We also constructed a novel amperometric glucose sensing system employing membrane-binding PQQ glucose dehydrogenase using the surface deposition method.