DECIPHERING THE IMPACT OF COLLAGENASE TREATMENT DURATION ON PRIMARY BREAST CANCER CELL PROTEOME: A COMPREHENSIVE STUDY

• Published in: Meandros medical and dental journal 2024-09, Vol.25 (3), p.222-232
• Main Authors: Bal Albayrak, Merve Gulsen, Kasap, Murat, Akpınar, Gürler, Yanar, Sevinc, Şimşek, Turgay, Cantürk, Zafer
• Format: Article
• Language: English
• ISSN: 2149-9063; 2149-9063
• DOI: 10.69601/meandrosmdj.1542417

Aim: Primary cell isolation is essential for studying cellular behavior and disease mechanisms, with collagenase-mediated tissue dissociation playing a critical role in the process. However, the impact of collagenase treatment duration on the proteome of primary cells, particularly in breast cancer research, remains underexplored. This study aims to investigate the effects of collagenase II treatment duration on the proteomic profiles of primary breast cancer cells. Materials and Methods: Breast cancer tissues from patients diagnosed with infiltrating ductal carcinoma were treated with collagenase II for either 1 or 3 hours. Subsequent proteomic analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Identified proteins were subjected to bioinformatic analyses to determine the functional implications of the proteomic changes induced by the different treatment durations. Results: Bioinformatic analyses showed that 1-hour treatment predominantly affected proteins involved in cytoskeletal organization and cell adhesion, with significant enrichment in actin cytoskeleton dynamics and structural molecule activity. In contrast, 3-hour treatment led to significant metabolic reprogramming, with enhanced regulation of pathways involved in energy production, including the TCA cycle and glycolysis. Conclusion: This study reveals for the first time that, collagenase II treatment duration significantly alters the proteomic profile of primary breast cancer cells, with shorter durations affecting structural proteins and longer durations inducing metabolic changes. Optimizing treatment time is crucial for targeted proteomic studies.