Association of HLA-DRB1, HLA-DQB1 Alleles, and TNF-α Promoter Polymorphisms with Multiple Sclerosis in the Cuban Population

• Published in: Bionatura Journal 2024-06, Vol.1 (2), p.1-12
• Main Authors: Cintado, A, Fernández-de-Cossío, ME, Nazabal, M, Díaz, T, Ale, M, Grass, D, Cervantes-Llanos, M, Pavon-Fuentes, N, Cabrera-Gomez, JA, Diaz de la Fe, A, Pentón-Rol, G
• Format: Article
• Language: English
• ISSN: 3020-7886; 3020-7886
• DOI: 10.70099/BJ/2024.02.01.14

Multiple sclerosis (MS) is an inflammatory, demyelinating, autoimmune disease of the central nervous system. It is known that the Major Histocompatibility Complex class II region produces the most potent effect on MS genetic susceptibility. In addition, the genetic polymorphism within the TNF locus has been involved in the pathogenesis of various autoimmune diseases. This study has the purpose of evaluating HLA-DRB1, HLA-DQB1 alleles and TNF promotor alpha gene polymorphism (SNP TNF- α -238 G/A; - 243G/A; -308 G/A; -375 G/A, -856 C/T; -862 C/A) in a sample of Cuban MS patients. Disease-associated HLA susceptibility alleles were genotyped by the SSP-PCR method. The TNF- α genotypes were identified by sequencing. The association was found between HLA and MS, DRB1*15:01, DRB1* 14:01, DQA*01:02 and DQB1*06:02 being susceptibility alleles. TNF-α-308 G (OR=1,6, P