RNA as an internal control for duplex RT-PCR assay of pea germplasm

Tobacco mosaic virus (TMV) is an easily prepared, plentiful and stable source of homogenous RNA suitable for addition to reverse transcription polymerase chain reaction (RT-PCR) assays as an internal control. We describe primers and conditions used in a duplex assay for Pea seedborne mosaic virus (P...

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Bibliographic Details
Published in:Australasian plant pathology 2001, Vol.30 (3), p.227-230
Main Author: V. A. Torok
Format: Article
Language:English
Online Access:Get full text
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Summary:Tobacco mosaic virus (TMV) is an easily prepared, plentiful and stable source of homogenous RNA suitable for addition to reverse transcription polymerase chain reaction (RT-PCR) assays as an internal control. We describe primers and conditions used in a duplex assay for Pea seedborne mosaic virus (PSbMV)-infection of pea seed as an example of the utility of the system for RT-PCR screening of plant germplasm for the presence or absence of a virus. Keywords: Pisum sativum, Potyvirus, PSbMV diagnostic RT-PCR primers, TMV RT-PCR primers. Australasian Plant Pathology 30(3) 227 - 230 Full text doi:10.1071/AP01028 © CSIRO 2001
ISSN:1448-6032
DOI:10.1071/AP01028