Structure of the DNA duplex d(ATTAAT)(2) with Hoogsteen hydrogen bonds

The traditional Watson-Crick base pairs in DNA may occasionally adopt a Hoogsteen conformation, with a different organization of hydrogen bonds. Previous crystal structures have shown that the Hoogsteen conformation is favored in alternating AT sequences of DNA. Here we present new data for a differ...

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Bibliographic Details
Main Authors: Acosta Reyes, Francisco Javier, Subirana, Juan Antonio, Campos López, Josefina de Lourdes, Alechaga, Elida
Format: Text Resource
Language:English
Subjects:
ADN
base-pairs
complex
crystal
DNA
Enginyeria química
Estructura
polymerase-iota
refinement
refmac5
replication
software
Synthesis
Àrees temàtiques de la UPC
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Summary:The traditional Watson-Crick base pairs in DNA may occasionally adopt a Hoogsteen conformation, with a different organization of hydrogen bonds. Previous crystal structures have shown that the Hoogsteen conformation is favored in alternating AT sequences of DNA. Here we present new data for a different sequence, d(ATTAAT)(2), which is also found in the Hoogsteen conformation. Thus we demonstrate that other all-AT sequences of DNA with a different sequence may be found in the Hoogsteen conformation. We conclude that any all-AT sequence might acquire this conformation under appropriate conditions. We also compare the detailed features of DNA in either the Hoogsteen or Watson-Crick conformations.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0120241