Coffee pulp supplementation prior to breeding improves oxidative status without affecting fertility of primiparous ewes

• Published in: Revista Colombiana de Ciencias Pecuarias 2019-10, Vol.32 (4), p.261-273
• Main Authors: Gutiérrez-Prado, Lizbet K, Sánchez-Torres-Esqueda, María T, Salinas-Rios, Teodulo, Figueroa-Velasco, José L, Martínez-Aispuro, José A, Contreras-Caro-Del-Castillo, David A, Cárdenas-León, Mario, Nava-Cuellar, Cuauhtémoc, Cordero-Mora, José L, García-Cue, José L
• Format: Article
• Language: English
• Subjects: antioxidant capacity; Antioxidants; Breeding; Breeding seasons; cafeína; caffeine; capacidad antioxidante; capacidade antioxidante; Coffee; coffee pulp; Coffee pulp as feed; Diet; estresse oxidativo; Estrus; estrus synchronization; estrés oxidativo; ewes; fertilidad; fertilidade; Fertility; fertility lipoperoxidation; Frequency analysis; Glucose; Insulin; Lipid peroxidation; Lipids; lipoperoxidación; lipoperoxidação; Oestrus synchronization; ovejas; ovelhas; ovinos; Oxidation; oxidative stress; parámetros reproductivos; parámetros reprodutivos; Peroxidación lipídica; Peroxidação lipídica; polpa de café; Polpa de café como ração para animais; Pregnancy; Progesterone; Prostaglandins; Pulp; pulpa de café; Pulpa de café como alimento para animales; Reproducción; Reproduction; reproductive parameters; Reprodução; sheep; Sincronización del celo; sincronización del estro; Sincronização do cio; sincronização do estro; Stress oxidativo; Synchronism; Synchronization
• ISSN: 0120-0690; 2256-2958
• DOI: 10.17533/udea.rccp.v32n4a02

Background: Coffee pulp has a high content of antioxidants capable of modifying the oxidative status in small ruminants. However, high amounts for a prolonged time can reduce fertility. Objective: To determine the effect of two inclusion levels of coffee pulp during estrous synchronization on reproductive variables and oxidative status of primiparous ewes. Methods: Sixty Suffolk x Dorset primiparous ewes were distributed into three treatments in a completely randomized design; T0: (n=20) 1.5 kg balanced diet, Tl: (n=20) 1.5 kg balanced diet and 5% coffee pulp, T2: (n=20) 1.5 kg balanced diet and 10% coffee pulp. Supplementation was given for 16 days before estrus synchronization and until the beginning of the breeding season. A progestogen (CIDR®) was inserted for ll days and a dose of PGF2a was applied two days prior to its withdrawal. Estrus detection started 12 hours after CIDR withdrawal. Blood samples were obtained during the supplementation period to measure oxidative status, antioxidant capacity, glucose and insulin, and up to 9 days after breeding to determine progesterone concentration. Pregnancy diagnosis was performed at 30 and 60 days post-breeding. An analysis of repeated measures of mixed effects and frequency analysis were carried out. Results: Inclusion of coffee pulp for a short period prior to breeding did not affect reproductive parameters, nor progesterone, glucose or insulin concentrations (p>0.05); however, antioxidant capacity increased, while lipid oxidation showed an opposite trend (p