A cross-sectional cohort study of the activity and turnover of neutrophil granulocytes in juvenile idiopathic arthritis

The inflammatory process in juvenile idiopathic arthritis (JIA) involves both the innate and the adaptive immune system. The turnover and activity of neutrophil granulocytes may be reflected by proteins secreted from primary or secondary granules and from the cytoplasm of sequestered cells. Our prim...

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Bibliographic Details
Published in:Pediatric Rheumatology 2021-06, Vol.19 (1), p.102-102, Article 102
Main Authors: Backlund, Malin, Venge, Per, Berntson, Lillemor
Format: Article
Language:English
Subjects:
Adolescent
Analysis
Arthritis
Arthritis, Juvenile - blood
Arthritis, Juvenile - immunology
Blood
Child
Child, Preschool
Cohort analysis
Cohort Studies
Cross-Sectional Studies
Enzymes
Female
Granule proteins
Granulocytes
Humans
Immune system
Inflammatory diseases
Juvenile idiopathic arthritis
Leukocyte L1 Antigen Complex - blood
Lipocalin-2 - blood
Male
Medical treatment
Neutrophil granulocytes
Neutrophils
Neutrophils - physiology
Pathophysiology
Patients
Pediatrics
Peroxidase - blood
Priming
Proteins
Rankings
Regression analysis
Rheumatoid arthritis
Rheumatology
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Summary:The inflammatory process in juvenile idiopathic arthritis (JIA) involves both the innate and the adaptive immune system. The turnover and activity of neutrophil granulocytes may be reflected by proteins secreted from primary or secondary granules and from the cytoplasm of sequestered cells. Our primary aim was to compare the levels of the secondary neutrophil granule protein human neutrophil lipocalin (HNL), in JIA patients and controls, and to explore a possible priming of neutrophils through parallel analyses in plasma and serum. A secondary aim was to relate the levels of HNL to two other well-studied leukocyte proteins, S100A8/A9 and myeloperoxidase (MPO), as well as to clinical aspects of JIA. The concentrations of the three biomarkers in serum, two of them also in plasma, were measured using enzyme-linked immunosorbent assay in 37 children with JIA without medical treatment, in high disease activity based on juvenile arthritis disease activity score 27 (JADAS27), 32 children on medical treatment, mainly in lower disease activity, and 16 healthy children. We assessed for differences between two groups using the Mann-Whitney U test, and used the Kruskal-Wallis test for multiple group comparisons. Spearman rank correlation, linear and multiple regression analyses were used for evaluation of associations between biomarker concentrations and clinical scores. The concentrations of HNL and MPO in serum were significantly increased in children with JIA (p 
ISSN:1546-0096
1546-0096
DOI:10.1186/s12969-021-00600-7