DNA flow cytometric analysis in variable types of hydropic placentas

• Published in: International journal of reproductive biomedicine (Yazd, Iran) Iran), 2015-05, Vol.13 (5), p.269-274
• Main Authors: Pasdar, Fatemeh Atabaki, Khooei, Alireza, Fazel, Alireza, Rastin, Maryam, Tabasi, Nafise, Peirouvi, Tahmineh, Mahmoudi, Mahmoud
• Format: Article
• Language: English
• Subjects: Flow cytometry; Hydatidiform mole; Original; Ploidy; Spontaneous abortion
• ISSN: 1680-6433; 2476-4108; 2476-3772; 2008-2177; 1680-6433

Background: Differential diagnosis between complete hydatidiform mole, partial hydatidiform mole and hydropic abortion, known as hydropic placentas is still a challenge for pathologists but it is very important for patient management. Objective: We analyzed the nuclear DNA content of various types of hydropic placentas by flowcytometry. Materials and Methods: DNA ploidy analysis was performed in 20 non-molar (hydropic and non-hydropic spontaneous abortions) and 20 molar (complete and partial moles), formalin-fixed, paraffin-embedded tissue samples by flow cytometry. The criteria for selection were based on the histopathologic diagnosis. Results: Of 10 cases histologically diagnosed as complete hydatiform mole, 9 cases yielded diploid histograms, and 1 case was tetraploid. Of 10 partial hydatidiform moles, 8 were triploid and 2 were diploid. All of 20 cases diagnosed as spontaneous abortions (hydropic and non-hydropic) yielded diploid histograms. Conclusion: These findings signify the importance of the combined use of conventional histology and ploidy analysis in the differential diagnosis of complete hydatidiform mole, partial hydatidiform mole and hydropic abortion.