Fine mapping of QYrsv.swust- 1BL for resistance to stripe rust in durum wheat Svevo

• Published in: Frontiers in plant science 2024-06, Vol.15, p.1395223
• Main Authors: Zhou, Xinli, Jia, Guoyun, Luo, Yuqi, Li, Xin, Cai, Lin, Chen, Xianming, Kang, Zhensheng
• Format: Article
• Language: English
• Subjects: durum wheat; fine mapping; Plant Science; quantitative trait locus; resistance gene; stripe rust
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2024.1395223

Stripe rust, caused by f. sp. ( ), is a serious disease that affects wheat worldwide. There is a great need to develop cultivars with combinations of all-stage resistance (ASR) and adult-plant resistance (APR) genes for sustainable control of the disease. in the Italian durum wheat ( ssp. ) cultivar Svevo is effective against races in China and Israel, and the gene has been previously mapped to the long arm of chromosome 1B. The gene is flanked by SNP (single nucleotide polymorphism) markers and (0.75 cM). In the present study, we used high-density 660K SNP array genotyping and the phenotypes of 137 recombinant inbred lines (RILs) to fine map the locus within a 1.066 Mb region in durum wheat Svevo (RefSeq Rel. 1.0) on chromosome arm 1BL. The identified 1.066 Mb region overlaps with a previously described map of , a stripe rust APR gene. Twenty-five candidate genes for were identified through comparing polymorphic genes within the 1.066 Mb region in the resistant cultivar. SNP markers were selected and converted to Kompetitive allele-specific polymerase chain reaction (KASP) markers. Five KASP markers based on SNP were validated in a F and F breeding population, providing further compelling evidence for the significant effects of . These markers should be useful in marker-assisted selection for incorporating into new durum and common wheat cultivars for resistance to stripe rust.