IN VITRO INTERACTION OF INFLUENZA VIRUS A(H1N1)pdm09 WITH MONOCYTIC MACROPHAGES: INDIVIDUAL RESPONSES OF TLR7 AND RIG1 RECEPTOR GENES

In vitro differentiation of donor blood monocytes to macrophages (Mph) following GM-CSF treatment was accompanied by a significant increase in the levels of gene transcription signaling receptors TLR7 or RIG1. The levels of intracellular viral RNA (M1 gene) in Mph remained high upon infection by inf...

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Bibliographic Details
Published in:Medit͡s︡inskai͡a︡ immunologii͡a 2017-12, Vol.19 (6), p.721-730
Main Authors: T. M. Sokolova, V. V. Poloskov, A. N. Shuvalov, I. A. Rudneva, T. A. Timofeeva
Format: Article
Language:Russian
Subjects:
gene expression tlr7
influenza virus a (h1n1)pdm09
monocytic macrophages
rig1 receptors
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Summary:In vitro differentiation of donor blood monocytes to macrophages (Mph) following GM-CSF treatment was accompanied by a significant increase in the levels of gene transcription signaling receptors TLR7 or RIG1. The levels of intracellular viral RNA (M1 gene) in Mph remained high upon infection by influenza virus A H1N1pdm (Moscow 2009) for 24-96 hours. The innate immunity reactions caused by influenza virus show individual features: they are decreased in Mph from donor 1 which had initially high level of endosomal TLR7 gene activity, and it increased by influenza virus in MPh from donor 2 who had a very low level of TLR7 gene expression. The influenza H1N1pdm virus weakly stimulated expression of gene RIG1 and production of inflammatory cytokines in Mf in donor 1. The differences may be connected with individual sensitivity of the donors to influenza infection.
ISSN:1563-0625
2313-741X
DOI:10.15789/1563-0625-2017-6-721-730