The transcription factor SoxD controls neuronal guidance in the Drosophila visual system

Precise control of neurite guidance during development is essential to ensure proper formation of neuronal networks and correct function of the central nervous system (CNS). How neuronal projections find their targets to generate appropriate synapses is not entirely understood. Although transcriptio...

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Bibliographic Details
Published in:Scientific reports 2018-09, Vol.8 (1), p.13332-12, Article 13332
Main Authors: Contreras, Esteban G., Palominos, Tomás, Glavic, Álvaro, Brand, Andrea H., Sierralta, Jimena, Oliva, Carlos
Format: Article
Language:English
Subjects:
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Axon guidance
Central nervous system
Drosophila
Ganglia
Glial cells
Humanities and Social Sciences
Insects
Medulla oblongata
multidisciplinary
Neural networks
Neural stem cells
Neurogenesis
Neuronal-glial interactions
Neuropil
Optic lobe
Optomotor response
RNA-mediated interference
Science
Science (multidisciplinary)
Stem cells
Synapses
Transcription factors
Visual system
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Summary:Precise control of neurite guidance during development is essential to ensure proper formation of neuronal networks and correct function of the central nervous system (CNS). How neuronal projections find their targets to generate appropriate synapses is not entirely understood. Although transcription factors are key molecules during neurogenesis, we do not know their entire function during the formation of networks in the CNS. Here, we used the Drosophila melanogaster optic lobe as a model for understanding neurite guidance during development. We assessed the function of Sox102F/SoxD, the unique Drosophila orthologue of the vertebrate SoxD family of transcription factors. SoxD is expressed in immature and mature neurons in the larval and adult lobula plate ganglia (one of the optic lobe neuropils), but is absent from glial cells, neural stem cells and progenitors of the lobula plate. SoxD RNAi knockdown in all neurons results in a reduction of the lobula plate neuropil, without affecting neuronal fate. This morphological defect is associated with an impaired optomotor response of adult flies. Moreover, knocking down SoxD only in T4/T5 neuronal types, which control motion vision, affects proper neurite guidance into the medulla and lobula. Our findings suggest that SoxD regulates neurite guidance, without affecting neuronal fate.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-31654-5