Loading…

Tea Polyphenol Inhibits Allostimulation in Mixed Lymphocyte Culture

Green tea polyphenols are known to protect allogenic donor tissues from acute rejection by their recipients. This immunosuppressive effect may be generated by a unique chemical property of the major component, epigallocatechin-o-gallate (EGCG), which can block specific cell surface molecules of the...

Full description

Saved in:
Bibliographic Details
Published in:Cell transplantation 2007-01, Vol.16 (1), p.75-83
Main Authors: Kim, Jong-Yoon, Kina, Tatsuo, Iwanaga, Yasuhiro, Noguchi, Hirofumi, Matsumura, Kazuaki, Hyon, Suong-Hyu
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c517t-a8d9c0cc115b8acf81ff2e413bccf71523b3c9149861c359c02e8d19d86d2a033
cites cdi_FETCH-LOGICAL-c517t-a8d9c0cc115b8acf81ff2e413bccf71523b3c9149861c359c02e8d19d86d2a033
container_end_page 83
container_issue 1
container_start_page 75
container_title Cell transplantation
container_volume 16
creator Kim, Jong-Yoon
Kina, Tatsuo
Iwanaga, Yasuhiro
Noguchi, Hirofumi
Matsumura, Kazuaki
Hyon, Suong-Hyu
description Green tea polyphenols are known to protect allogenic donor tissues from acute rejection by their recipients. This immunosuppressive effect may be generated by a unique chemical property of the major component, epigallocatechin-o-gallate (EGCG), which can block specific cell surface molecules of the donor tissues. To test this hypothesis, we examined the effects of EGCG on the murine mixed lymphocyte reactions. EGCG treatment of stimulator cells significantly attenuated the proliferation of responder T cells. The proliferation did not recover upon the secondary stimulations by fresh untreated cells or exogenous IL-2. Flow cytometric analyses showed that EGCG treatment decreased the staining intensities of various cell surface molecules including MHC II, which plays a major role in antigen presentation, and B7.1, B7.2, and their ligand, CD28, which are required for costimulatory signals in T-cell activation. These results suggest that an anergic state of alloreactive T cells may be induced by either weakening of antigen signaling or blockage of costimulatory signals with EGCG. Other possible mechanisms behind the immunosuppressive effect and a potential use of EGCG treatment of donor tissues in transplantation medicine are discussed.
doi_str_mv 10.3727/000000007783464515
format article
fullrecord <record><control><sourceid>proquest_AFRWT</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_00461222c7bb47a1824cac764697c12e</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.3727_000000007783464515</sage_id><doaj_id>oai_doaj_org_article_00461222c7bb47a1824cac764697c12e</doaj_id><sourcerecordid>70387267</sourcerecordid><originalsourceid>FETCH-LOGICAL-c517t-a8d9c0cc115b8acf81ff2e413bccf71523b3c9149861c359c02e8d19d86d2a033</originalsourceid><addsrcrecordid>eNp9kU1r3DAQhkVpaDYff6CH4lNvbjT69jEsTbuwpTmkZyGP5awW2dpKNnT_fZ3u0h4CncvA8MwzMC8h74F-4prpO3ourQ0XSkiQb8gKpJQ1Nw17S1a0UbxWptGX5KqU_QvKmXxHLkELrozUK7J-8q56TPF42PkxxWoz7kIbplLdx5jKFIY5uimksQpj9S388l21PQ6HXcLj5Kv1HKc5-xty0btY_O25X5MfD5-f1l_r7fcvm_X9tkYJeqqd6RqkiACyNQ57A33PvADeIvYaJOMtxwZEYxQglwvLvOmg6YzqmKOcX5PNydslt7eHHAaXjza5YP8MUn62Lk8Bo7eUCgWMMdRtK7QDwwQ61EqoRiMwv7g-nlyHnH7Ovkx2CAV9jG70aS5WU240U3oB2QnEnErJvv97GKh9icG-jmFZ-nC2z-3gu38r578vwN0JKO7Z232a87h87n_K3z62jn8</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70387267</pqid></control><display><type>article</type><title>Tea Polyphenol Inhibits Allostimulation in Mixed Lymphocyte Culture</title><source>SAGE database</source><creator>Kim, Jong-Yoon ; Kina, Tatsuo ; Iwanaga, Yasuhiro ; Noguchi, Hirofumi ; Matsumura, Kazuaki ; Hyon, Suong-Hyu</creator><creatorcontrib>Kim, Jong-Yoon ; Kina, Tatsuo ; Iwanaga, Yasuhiro ; Noguchi, Hirofumi ; Matsumura, Kazuaki ; Hyon, Suong-Hyu</creatorcontrib><description>Green tea polyphenols are known to protect allogenic donor tissues from acute rejection by their recipients. This immunosuppressive effect may be generated by a unique chemical property of the major component, epigallocatechin-o-gallate (EGCG), which can block specific cell surface molecules of the donor tissues. To test this hypothesis, we examined the effects of EGCG on the murine mixed lymphocyte reactions. EGCG treatment of stimulator cells significantly attenuated the proliferation of responder T cells. The proliferation did not recover upon the secondary stimulations by fresh untreated cells or exogenous IL-2. Flow cytometric analyses showed that EGCG treatment decreased the staining intensities of various cell surface molecules including MHC II, which plays a major role in antigen presentation, and B7.1, B7.2, and their ligand, CD28, which are required for costimulatory signals in T-cell activation. These results suggest that an anergic state of alloreactive T cells may be induced by either weakening of antigen signaling or blockage of costimulatory signals with EGCG. Other possible mechanisms behind the immunosuppressive effect and a potential use of EGCG treatment of donor tissues in transplantation medicine are discussed.</description><identifier>ISSN: 0963-6897</identifier><identifier>EISSN: 1555-3892</identifier><identifier>DOI: 10.3727/000000007783464515</identifier><identifier>PMID: 17436857</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Apoptosis ; Catechin - analogs &amp; derivatives ; Catechin - pharmacology ; Cell Proliferation ; Cells, Cultured ; Female ; Flavonoids - pharmacology ; Flow Cytometry ; Immunosuppressive Agents - pharmacology ; Interleukin-2 - immunology ; Lymphocyte Activation - drug effects ; Lymphocyte Culture Test, Mixed ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Phenols - pharmacology ; Plant Extracts - chemistry ; Polyphenols ; Tea - chemistry</subject><ispartof>Cell transplantation, 2007-01, Vol.16 (1), p.75-83</ispartof><rights>2007 Cognizant Comm. Corp.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-a8d9c0cc115b8acf81ff2e413bccf71523b3c9149861c359c02e8d19d86d2a033</citedby><cites>FETCH-LOGICAL-c517t-a8d9c0cc115b8acf81ff2e413bccf71523b3c9149861c359c02e8d19d86d2a033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.3727/000000007783464515$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.3727/000000007783464515$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,4024,21966,27853,27923,27924,27925,44945,45333</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.3727/000000007783464515?utm_source=summon&amp;utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17436857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Jong-Yoon</creatorcontrib><creatorcontrib>Kina, Tatsuo</creatorcontrib><creatorcontrib>Iwanaga, Yasuhiro</creatorcontrib><creatorcontrib>Noguchi, Hirofumi</creatorcontrib><creatorcontrib>Matsumura, Kazuaki</creatorcontrib><creatorcontrib>Hyon, Suong-Hyu</creatorcontrib><title>Tea Polyphenol Inhibits Allostimulation in Mixed Lymphocyte Culture</title><title>Cell transplantation</title><addtitle>Cell Transplant</addtitle><description>Green tea polyphenols are known to protect allogenic donor tissues from acute rejection by their recipients. This immunosuppressive effect may be generated by a unique chemical property of the major component, epigallocatechin-o-gallate (EGCG), which can block specific cell surface molecules of the donor tissues. To test this hypothesis, we examined the effects of EGCG on the murine mixed lymphocyte reactions. EGCG treatment of stimulator cells significantly attenuated the proliferation of responder T cells. The proliferation did not recover upon the secondary stimulations by fresh untreated cells or exogenous IL-2. Flow cytometric analyses showed that EGCG treatment decreased the staining intensities of various cell surface molecules including MHC II, which plays a major role in antigen presentation, and B7.1, B7.2, and their ligand, CD28, which are required for costimulatory signals in T-cell activation. These results suggest that an anergic state of alloreactive T cells may be induced by either weakening of antigen signaling or blockage of costimulatory signals with EGCG. Other possible mechanisms behind the immunosuppressive effect and a potential use of EGCG treatment of donor tissues in transplantation medicine are discussed.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Catechin - analogs &amp; derivatives</subject><subject>Catechin - pharmacology</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Flavonoids - pharmacology</subject><subject>Flow Cytometry</subject><subject>Immunosuppressive Agents - pharmacology</subject><subject>Interleukin-2 - immunology</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Phenols - pharmacology</subject><subject>Plant Extracts - chemistry</subject><subject>Polyphenols</subject><subject>Tea - chemistry</subject><issn>0963-6897</issn><issn>1555-3892</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9kU1r3DAQhkVpaDYff6CH4lNvbjT69jEsTbuwpTmkZyGP5awW2dpKNnT_fZ3u0h4CncvA8MwzMC8h74F-4prpO3ourQ0XSkiQb8gKpJQ1Nw17S1a0UbxWptGX5KqU_QvKmXxHLkELrozUK7J-8q56TPF42PkxxWoz7kIbplLdx5jKFIY5uimksQpj9S388l21PQ6HXcLj5Kv1HKc5-xty0btY_O25X5MfD5-f1l_r7fcvm_X9tkYJeqqd6RqkiACyNQ57A33PvADeIvYaJOMtxwZEYxQglwvLvOmg6YzqmKOcX5PNydslt7eHHAaXjza5YP8MUn62Lk8Bo7eUCgWMMdRtK7QDwwQ61EqoRiMwv7g-nlyHnH7Ovkx2CAV9jG70aS5WU240U3oB2QnEnErJvv97GKh9icG-jmFZ-nC2z-3gu38r578vwN0JKO7Z232a87h87n_K3z62jn8</recordid><startdate>200701</startdate><enddate>200701</enddate><creator>Kim, Jong-Yoon</creator><creator>Kina, Tatsuo</creator><creator>Iwanaga, Yasuhiro</creator><creator>Noguchi, Hirofumi</creator><creator>Matsumura, Kazuaki</creator><creator>Hyon, Suong-Hyu</creator><general>SAGE Publications</general><general>SAGE Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>200701</creationdate><title>Tea Polyphenol Inhibits Allostimulation in Mixed Lymphocyte Culture</title><author>Kim, Jong-Yoon ; Kina, Tatsuo ; Iwanaga, Yasuhiro ; Noguchi, Hirofumi ; Matsumura, Kazuaki ; Hyon, Suong-Hyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-a8d9c0cc115b8acf81ff2e413bccf71523b3c9149861c359c02e8d19d86d2a033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Catechin - analogs &amp; derivatives</topic><topic>Catechin - pharmacology</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Flavonoids - pharmacology</topic><topic>Flow Cytometry</topic><topic>Immunosuppressive Agents - pharmacology</topic><topic>Interleukin-2 - immunology</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocyte Culture Test, Mixed</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Phenols - pharmacology</topic><topic>Plant Extracts - chemistry</topic><topic>Polyphenols</topic><topic>Tea - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jong-Yoon</creatorcontrib><creatorcontrib>Kina, Tatsuo</creatorcontrib><creatorcontrib>Iwanaga, Yasuhiro</creatorcontrib><creatorcontrib>Noguchi, Hirofumi</creatorcontrib><creatorcontrib>Matsumura, Kazuaki</creatorcontrib><creatorcontrib>Hyon, Suong-Hyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cell transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Kim, Jong-Yoon</au><au>Kina, Tatsuo</au><au>Iwanaga, Yasuhiro</au><au>Noguchi, Hirofumi</au><au>Matsumura, Kazuaki</au><au>Hyon, Suong-Hyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tea Polyphenol Inhibits Allostimulation in Mixed Lymphocyte Culture</atitle><jtitle>Cell transplantation</jtitle><addtitle>Cell Transplant</addtitle><date>2007-01</date><risdate>2007</risdate><volume>16</volume><issue>1</issue><spage>75</spage><epage>83</epage><pages>75-83</pages><issn>0963-6897</issn><eissn>1555-3892</eissn><abstract>Green tea polyphenols are known to protect allogenic donor tissues from acute rejection by their recipients. This immunosuppressive effect may be generated by a unique chemical property of the major component, epigallocatechin-o-gallate (EGCG), which can block specific cell surface molecules of the donor tissues. To test this hypothesis, we examined the effects of EGCG on the murine mixed lymphocyte reactions. EGCG treatment of stimulator cells significantly attenuated the proliferation of responder T cells. The proliferation did not recover upon the secondary stimulations by fresh untreated cells or exogenous IL-2. Flow cytometric analyses showed that EGCG treatment decreased the staining intensities of various cell surface molecules including MHC II, which plays a major role in antigen presentation, and B7.1, B7.2, and their ligand, CD28, which are required for costimulatory signals in T-cell activation. These results suggest that an anergic state of alloreactive T cells may be induced by either weakening of antigen signaling or blockage of costimulatory signals with EGCG. Other possible mechanisms behind the immunosuppressive effect and a potential use of EGCG treatment of donor tissues in transplantation medicine are discussed.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>17436857</pmid><doi>10.3727/000000007783464515</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier ISSN: 0963-6897
ispartof Cell transplantation, 2007-01, Vol.16 (1), p.75-83
issn 0963-6897
1555-3892
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_00461222c7bb47a1824cac764697c12e
source SAGE database
subjects Animals
Apoptosis
Catechin - analogs & derivatives
Catechin - pharmacology
Cell Proliferation
Cells, Cultured
Female
Flavonoids - pharmacology
Flow Cytometry
Immunosuppressive Agents - pharmacology
Interleukin-2 - immunology
Lymphocyte Activation - drug effects
Lymphocyte Culture Test, Mixed
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Phenols - pharmacology
Plant Extracts - chemistry
Polyphenols
Tea - chemistry
title Tea Polyphenol Inhibits Allostimulation in Mixed Lymphocyte Culture
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T12%3A38%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_AFRWT&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tea%20Polyphenol%20Inhibits%20Allostimulation%20in%20Mixed%20Lymphocyte%20Culture&rft.jtitle=Cell%20transplantation&rft.au=Kim,%20Jong-Yoon&rft.date=2007-01&rft.volume=16&rft.issue=1&rft.spage=75&rft.epage=83&rft.pages=75-83&rft.issn=0963-6897&rft.eissn=1555-3892&rft_id=info:doi/10.3727/000000007783464515&rft_dat=%3Cproquest_AFRWT%3E70387267%3C/proquest_AFRWT%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c517t-a8d9c0cc115b8acf81ff2e413bccf71523b3c9149861c359c02e8d19d86d2a033%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=70387267&rft_id=info:pmid/17436857&rft_sage_id=10.3727_000000007783464515&rfr_iscdi=true