Bee Venom Phospholipase A2 Ameliorates House Dust Mite Extract Induced Atopic Dermatitis Like Skin Lesions in Mice

• Published in: Toxins 2017-02, Vol.9 (2), p.68-68
• Main Authors: Jung, Kyung-Hwa, Baek, Hyunjung, Kang, Manho, Kim, Namsik, Lee, Seung Young, Bae, Hyunsu
• Format: Article
• Language: English
• Subjects: Animals; Anti-Allergic Agents - isolation & purification; Anti-Allergic Agents - pharmacology; Asthma; atopic dermatitis; Bee Venoms - enzymology; bvPLA2; Chemotaxis, Leukocyte - drug effects; Cytokines; Cytokines - immunology; Cytokines - metabolism; Depletion; Dermatitis; Dermatitis, Atopic - blood; Dermatitis, Atopic - drug therapy; Dermatitis, Atopic - immunology; Dermatitis, Atopic - pathology; Dermatophagoides farinae - immunology; Dermatophagoides pteronyssinus; Dinitrochlorobenzene; Disease Models, Animal; Dust; Ear; Eczema; House dust; IgE; Immune system; Immunoglobulin E - blood; Immunological tolerance; Immunology; Infiltration; Inflammation; Insect Proteins - isolation & purification; Insect Proteins - pharmacology; Lesions; Lymphocytes; Lymphocytes T; mast cell; Mast Cells - drug effects; Mast Cells - immunology; Mast Cells - metabolism; Mice; Mice, Inbred BALB C; Mites; Phospholipase; Phospholipases A2 - isolation & purification; Phospholipases A2 - pharmacology; Signs and symptoms; Skin - drug effects; Skin - immunology; Skin - metabolism; Skin - pathology; Skin diseases; skin lesion; T-Lymphocytes, Regulatory - drug effects; T-Lymphocytes, Regulatory - immunology; T-Lymphocytes, Regulatory - metabolism; Th1 Cells - drug effects; Th1 Cells - immunology; Th1 Cells - metabolism; Th2 Cells - drug effects; Th2 Cells - immunology; Th2 Cells - metabolism; Thickness; Time Factors
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins9020068

Atopic dermatitis (AD) is a biphasic inflammatory skin disease that is provoked by epidermal barrier defects, immune dysregulation, and increased skin infections. Previously, we have demonstrated that bvPLA2 evoked immune tolerance by inducing regulatory T cells (Treg), and thus alleviated Th2 dominant allergic asthma in mice. Here, we would like to determine whether treatment with bvPLA2 exacerbates the AD-like allergic inflammations induced by house dust mite extract (DFE) in a murine model. Epidermal thickness, immune cell infiltration, serum immunoglobulin, and cytokines were measured. Ear swelling, skin lesions, and the levels of total serum IgE and Th1/Th2 cytokines were elevated in DFE/DNCB-induced AD mice. Topical application of bvPLA2 elicited significant suppression of the increased AD symptoms, including ear thickness, serum IgE concentration, inflammatory cytokines, and histological changes. Furthermore, bvPLA2 treatment inhibited mast cell infiltration into the ear. On the other hand, Treg cell depletion abolished the anti-atopic effects of bvPLA2, suggesting that the effects of bvPLA2 depend on the existence of Tregs. Taken together, the results revealed that topical exposure to bvPLA2 aggravated atopic skin inflammation, suggesting that bvPLA2 might be a candidate for the treatment of AD.