SCC3 is an axial element essential for homologous chromosome pairing and synapsis

Cohesin is a multi-subunit protein that plays a pivotal role in holding sister chromatids together during cell division. Sister chromatid cohesion 3 (SCC3), constituents of cohesin complex, is highly conserved from yeast to mammals. Since the deletion of individual cohesin subunit always causes leth...

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Published in:eLife 2024-06, Vol.13
Main Authors: Zhao, Yangzi, Ren, Lijun, Zhao, Tingting, You, Hanli, Miao, Yongjie, Liu, Huixin, Cao, Lei, Wang, Bingxin, Shen, Yi, Li, Yafei, Tang, Ding, Cheng, Zhukuan
Format: Article
Language:English
Subjects:
Cell Biology
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell division
Chromatids
Chromatin
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Chromosome deletion
Chromosome Pairing
Chromosomes
Cohesin
Cohesins
CRISPR
CRISPR-Cas Systems
Gene expression
Genetics and Genomics
Lethality
Localization
meiocyte
Meiosis
Meiosis - genetics
Mitosis
Morphology
Mutants
Oryza - genetics
Prophase
Proteins
rice
Sister chromatids
Sterility
Synaptonemal complex
Synaptonemal Complex - genetics
Synaptonemal Complex - metabolism
Yeast
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Summary:Cohesin is a multi-subunit protein that plays a pivotal role in holding sister chromatids together during cell division. Sister chromatid cohesion 3 (SCC3), constituents of cohesin complex, is highly conserved from yeast to mammals. Since the deletion of individual cohesin subunit always causes lethality, it is difficult to dissect its biological function in both mitosis and meiosis. Here, we obtained weak mutants using CRISPR-Cas9 system to explore its function during rice mitosis and meiosis. The weak mutants displayed obvious vegetative defects and complete sterility, underscoring the essential roles of SCC3 in both mitosis and meiosis. SCC3 is localized on chromatin from interphase to prometaphase in mitosis. However, in meiosis, SCC3 acts as an axial element during early prophase I and subsequently situates onto centromeric regions following the disassembly of the synaptonemal complex. The loading of SCC3 onto meiotic chromosomes depends on REC8. shows severe defects in homologous pairing and synapsis. Consequently, SCC3 functions as an axial element that is essential for maintaining homologous chromosome pairing and synapsis during meiosis.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.94180