An ethanol extract of Poria cocos inhibits the proliferation of non-small cell lung cancer A549 cells via the mitochondria-mediated caspase activation pathway

• Published in: Journal of functional foods 2016-05, Vol.23, p.614-627
• Main Authors: Chu, Bo-Feng, Lin, Hang-Ching, Huang, Xin-Wen, Huang, Han-Yu, Wu, Carol P., Kao, Ming-Ching
• Format: Article
• Language: English
• Subjects: Anti-tumour; Apoptosis; Cytotoxicity; Non-small cell lung cancer; Poria cocos; Tumulosic acid
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2016.03.016

•An ethanol extract prepared from fungus Poria cocos induces apoptosis in A549 cells.•Apoptosis is triggered via mitochondria-mediated caspase activation pathway.•The extract may serve as a food supplement for non-small cell lung cancer patients. Poria cocos, a food ingredient and a traditional Chinese medicine, is a fungus commonly used in eastern countries. It exhibits diuretic, anti-inflammatory, immunomodulatory and anti-hyperglycaemic functions. In addition, the polysaccharide and triterpenoid components isolated from P. cocos exhibit anti-tumour effects in a number of cancer cell lines. Here, an extract of P. cocos, designated H287, was evaluated for its anti-lung cancer potential. H287 induced apoptosis in A549 cells via the mitochondria-mediated caspase activation pathway, as demonstrated by the loss of mitochondria outer membrane potential, release of cytochrome c into the cytosol, and the cleavage of procapsase-9 to caspase-9. Further analyses suggest that tumulosic acid, one of the triterpenoids present in H287, is responsible for the cytotoxic function of H287. H287 may potentially serve as a dietary supplement or functional food ingredient for patients suffering from non-small cell lung cancers.