The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in Chlamydomonas reinhardtii

The synchronous triacylglycerol (TAG) production with the growth is a key step to lower the cost of the microalgae-based biofuel production. Phospholipid: diacylglycerol acyltransferase (PDAT) has been identified recently and catalyzes the phospholipid contributing acyl group to diacylglycerol to sy...

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Bibliographic Details
Published in:Biotechnology for biofuels 2018-06, Vol.11 (1), p.156-156, Article 156
Main Authors: Zhu, Zhen, Yuan, Guangze, Fan, Xuran, Fan, Yan, Yang, Miao, Yin, Yalei, Liu, Jiao, Liu, Yang, Cao, Xupeng, Tian, Jing, Xue, Song
Format: Article
Language:English
Subjects:
Accumulation
Acyltransferase
Algae
Arabidopsis
Biodiesel fuels
Biofuels
Biosynthesis
Cell growth
Chemical synthesis
Chlamydomonas
Chlamydomonas reinhardtii
Chloroplasts
Cloning
Cultivation
Diacylglyceryltrimethylhomoserine
Diglycerides
E coli
Enzymes
Fatty acids
Fungi
Gene expression
Genetic aspects
Genetic engineering
Genomics
Growth
Linum usitatissimum
Lipid
Lipids
Metabolism
PDAT
Phospholipid: diacylglycerol acyltransferase
Phospholipids
Physiological aspects
Plasmids
Production processes
Saccharomyces cerevisiae
Synchronous TAG production
Tetraselmis
Triglycerides
Variation
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Summary:The synchronous triacylglycerol (TAG) production with the growth is a key step to lower the cost of the microalgae-based biofuel production. Phospholipid: diacylglycerol acyltransferase (PDAT) has been identified recently and catalyzes the phospholipid contributing acyl group to diacylglycerol to synthesize TAG, and is considered as the important source of TAG in . Using a chimeric Hsp70A-RbcS2 promoter, exogenous PDAT form fused with a chloroplast transit peptide was expressed in CC-137. Proved by western blot, the expression of ScPDAT showed a synchronous trend to the growth in the exponential phase. Compared to the wild type, the strain of achieved 22% increase in the content of total fatty acids and 32% increase in TAG content. In addition, the fluctuation of C16 series fatty acid in monogalactosyldiacylglycerol, diacylglyceryltrimethylhomoserine and TAG indicated an enhancement in the TAG accumulation pathway. The TAG production was enhanced in the regular cultivation without the nutrient stress by strengthening the conversion of polar lipid to TAG in and the findings provide a candidate strategy for rational engineered strain to overcome the decline in the growth during the TAG accumulation triggered by nitrogen starvation.
ISSN:1754-6834
1754-6834
DOI:10.1186/s13068-018-1160-6