Role of Lipid-Based and Polymer-Based Non-Viral Vectors in Nucleic Acid Delivery for Next-Generation Gene Therapy

The field of gene therapy has experienced an insurgence of attention for its widespread ability to regulate gene expression by targeting genomic DNA, messenger RNA, microRNA, and short-interfering RNA for treating malignant and non-malignant disorders. Numerous nucleic acid analogs have been develop...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2020-06, Vol.25 (12), p.2866
Main Authors: Wahane, Aniket, Waghmode, Akaash, Kapphahn, Alexander, Dhuri, Karishma, Gupta, Anisha, Bahal, Raman
Format: Article
Language:English
Subjects:
Acids
Analogs
Ataxia
Cationic polymerization
Deoxyribonucleic acid
Design of experiments
DNA
DNA - genetics
DNA - therapeutic use
Efficiency
Ethanol
Expression vectors
Gene expression
Gene regulation
Gene therapy
Gene Transfer Techniques
Genetic Therapy - trends
Genetic Vectors - genetics
Genetic Vectors - therapeutic use
Genomes
Humans
lipid nanoparticles
Lipids
Lipids - genetics
Lipids - therapeutic use
Liposomes
miRNA
mRNA
Nanoparticles
Nanoparticles - chemistry
Nucleic acids
Physiology
Polyethylene glycol
Polymers
Polymers - chemistry
Polymers - therapeutic use
Proteins
Review
RNA, Small Interfering - genetics
RNA, Small Interfering - therapeutic use
Therapeutic applications
Vectors (Biology)
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Summary:The field of gene therapy has experienced an insurgence of attention for its widespread ability to regulate gene expression by targeting genomic DNA, messenger RNA, microRNA, and short-interfering RNA for treating malignant and non-malignant disorders. Numerous nucleic acid analogs have been developed to target coding or non-coding sequences of the human genome for gene regulation. However, broader clinical applications of nucleic acid analogs have been limited due to their poor cell or organ-specific delivery. To resolve these issues, non-viral vectors based on nanoparticles, liposomes, and polyplexes have been developed to date. This review is centered on non-viral vectors mainly comprising of cationic lipids and polymers for nucleic acid-based delivery for numerous gene therapy-based applications.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules25122866